Evidence map›Paper›PMID 28288813›Full record

ArticleBiochimica et biophysica acta. General subjects2017

A genomically modified Escherichia coli strain carrying an orthogonal E. coli histidyl-tRNA synthetase•tRNA

Markus Englert, Oscar Vargas-Rodriguez, Noah M Reynolds, Yane-Shih Wang, Dieter Söll, Takuya Umehara

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochimica et biophysica acta. General subjects, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Non-canonical Amino Acid Substrates of E. coli Aminoacyl-tRNA Synthetases.Chembiochem : a European journal of chemical biology · 2022
    Review
  3. Rational Design of Aptamer-Tagged tRNAs.International journal of molecular sciences · 2020
    Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Markus EnglertDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.
Oscar Vargas-RodriguezDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.
Noah M ReynoldsDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.
Yane-Shih WangDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.
Dieter SöllDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA; Department of Chemistry, Yale University, New Haven, CT 06520, USA. Electronic address: dieter.soll@yale.edu.
Takuya UmeharaDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA; Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan. Electronic address: tumehara@rs.noda.tus.ac.jp.
Yale University · USTokyo University of Science · JP

Funding

TRANSFER RNAR01GM022854 · NIGMS · YALE UNIVERSITY · PI SOLL, DIETER · 1985 to 2016
$21.3M
Studies of Transfer RNAR35GM122560 · NIGMS · YALE UNIVERSITY · PI SOLL, DIETER · 2017 to 2022
$5.6M
TRANSFER RNAR37GM022854 · NIGMS · YALE UNIVERSITY · PI SOLL, DIETER G · 1991 to 2000
$786k
NIGMS NIH HHS R01 GM022854NIGMS NIH HHS R35 GM122560NIGMS NIH HHS R37 GM022854
6 · The paper itself

Abstract

backgroundDevelopment of new aminoacyl-tRNA synthetase (aaRS)•tRNA pairs is central for incorporation of novel non-canonical amino acids (ncAAs) into proteins via genetic code expansion (GCE). The Escherichia coli and Caulobacter crescentus histidyl-tRNA synthetases (HisRS) evolved divergent mechanisms of tRNA

methodsE. coli was genetically engineered to use a C. crescentus HisRS•tRNA

resultsA genomically modified E. coli strain (named MEOV1) was created. MEVO1 requires an active C. crescentus HisRS•tRNA

conclusionsThe C. crescentus HisRS•tRNA GENERAL SIGNIFICANCE: We developed a platform that allows protein engineering of E. coli HisRS that should facilitate GCE in E. coli. This article is part of a Special Issue entitled "Biochemistry of Synthetic Biology - Recent Developments" Guest Editor: Dr. Ilka Heinemann and Dr. Patrick O'Donoghue.

Indexed as

Cloning, MolecularEscherichia coliGene LibraryGenetic EngineeringHistidine-tRNA LigaseMutagenesis, Site-DirectedProtein EngineeringRNA, Transfer, HisHistidine-tRNA LigaseRNA, Transfer, HisAminoacyl-tRNA synthetaseGenetic code expansionNon-canonical amino acidsOrthogonal pairSynthetic biologytRNA

Identifiers

PMID28288813
PMCPMC5592127
OpenAlexW2592355482

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.