ArticleParasites & vectors2017
Histological and parasitological distinctive findings in clinically-lesioned and normal-looking skin of dogs with different clinical stages of leishmaniosis.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- World Association for Veterinary Dermatology Consensus Statement for Diagnosis, and Evidence-Based Clinical Practice Guidelines for Treatment and Prevention of Canine Leishmaniosis.Veterinary dermatology · 2025Guideline
- LeishVet update and current recommendations on feline leishmaniosis.Parasites & vectors · 2026Review
- Updated Canine Leishmaniosis Working Group recommendations for leishmaniosis in dogs: Q&A on clinical management.Parasites & vectors · 2026Review
- Detection of Leishmania infantum DNA in captive wild mammals from an urban zoo in a visceral leishmaniasis-endemic area of Brazil.PLoS neglected tropical diseases · 2026Article
- Importance of Different Parameters for Monitoring Dogs withPathogens (Basel, Switzerland) · 2025Article
- When Precision Matters: Bone Marrow Cytology Meets qPCR in a Pilot Study QuantifyingMicroorganisms · 2025Article
- Article
- Clinical anemia predicts dermal parasitism and reservoir infectiousness during progressive visceral leishmaniosis.PLoS neglected tropical diseases · 2024Article
- Anatomical Vascular Differences andPathogens (Basel, Switzerland) · 2024Article
- First Report of Autochthonous Canine Leishmaniasis in Hong Kong.Microorganisms · 2022Article
- Detection of specific antibodies against Leishmania infantum in canine serum and oral transudate using an in-house ELISA.Parasites & vectors · 2022Article
- Comparison of immunohistochemical and qPCR methods from granulomatous dermatitis lesions for detection of leishmania in dogs living in endemic areas: a preliminary study.Parasites & vectors · 2022Article
- In and out:Frontiers in cellular and infection microbiology · 2022Article
- Toll-Like Receptors 2, 4, and 7, Interferon-Gamma, Interleukin 10, and Programmed Death Ligand 1 Transcripts in Leishmanin Skin Test-Positive Reactions of Ibizan Hound Dogs.Journal of immunology research · 2020Article
- Toll-like receptors 2, 4 and 7, interferon-gamma and interleukin 10, and programmed death ligand 1 transcripts in skin from dogs of different clinical stages of leishmaniosis.Parasites & vectors · 2019Article
- Parasite Specific Antibody Levels, Interferon-γ and TLR2 and TLR4 Transcripts in Blood from Dogs with Different Clinical Stages of Leishmaniosis.Veterinary sciences · 2018Article
- Serum Symmetric Dimethylarginine as an Early Marker of Excretory Dysfunction in Canine LeishmaniosisVeterinary medicine international · 2018Article
- Canine Leishmaniasis: An Overview of the Current Status and Strategies for Control.BioMed research international · 2018Review
- The evolution of trypanosomatid taxonomy.Parasites & vectors · 2017Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNormal-looking skin of dogs with leishmaniosis frequently shows microscopic lesions along with the presence of Leishmania amastigotes. However, histological lesions with or without detection of amastigotes might not occur in less severe clinical cases. In addition, comparative studies between paired clinically-lesioned and normal-looking skin samples from dogs with different disease severity are lacking. The objective of this study was to compare histological and parasitological findings by Leishmania immunohistochemistry (IHC) and quantitative PCR (qPCR) on paired clinically-lesioned and normal-looking skin biopsies from 25 dogs with different clinical stages of leishmaniosis, 11 with stage I-mild disease (papular dermatitis) and 14 with stage II-III (ulcerative or exfoliative dermatitis).
resultsThe study demonstrated microscopic lesions in 14 out of 25 (56%) samples from normal-looking skin biopsies. In those samples, perivascular to interstitial dermatitis composed by macrophages with lymphocytes and plasma cells was observed mainly in the superficial and mid-dermis. The intensity of the dermatitis was mild to moderate and always less prominent than in the clinically-lesioned skin. In normal-looking skin samples, the presence of parasites was detected by histology, IHC and qPCR in 5/25 (20%), 8/25 (32%) and 18/25 (72%), respectively. Leishmania was encountered in 11/25 (44%), 23/25 (92%) and 25/25 (100%) of clinically-lesioned skin samples by histology, IHC and qPCR, respectively. Normal-looking skin from dogs with stage I-mild disease was less frequently inflamed (P = 0.0172). Furthermore, Leishmania was more easily demonstrated by histology (P = 0.0464), IHC (P = 0.0421) or qPCR (P = 0.0068) in normal-looking skin of dogs with stage II-III-moderate to severe disease. In addition, in the latter group, there was a significantly higher parasite load studied by means of qPCR than in dogs with less severe disease (P = 0.043). Clinically-lesioned skin from dogs with stage I disease was more frequently characterised by the nodular to diffuse pattern and granuloma formation (P = 0.0166) and by a lower parasite load studied by means of qPCR (P = 0.043) compared with more diseased dogs.
conclusionsNormal-looking skin from dogs with stage I is less likely to present histological lesions as well as harbour the parasite when compared with dogs with moderate to severe leishmaniosis.
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