ArticleMicrobial cell factories2017
Functional differentiation of 3-ketosteroid Δ
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Degradation of CCurrent microbiology · 2025Article
- Improving the production of 9α-hydroxy-4-androstene-3,17-dione from phytosterols by 3-ketosteroid-ΔMicrobial cell factories · 2023Article
- Structure, Mutagenesis, and QM:MM Modeling of 3-Ketosteroid ΔBiochemistry · 2023Article
- A New 3-Ketosteroid-ΔMicroorganisms · 2022Article
- Steroid Metabolism in Thermophilic ActinobacteriumMicroorganisms · 2021Article
- Further Studies on the 3-Ketosteroid 9α-Hydroxylase ofMicroorganisms · 2021Article
- Diversity of Plant Sterols Metabolism: The Impact on Human Health, Sport, and Accumulation of Contaminating Sterols.Nutrients · 2021Review
- Different genome-wide transcriptome responses of Nocardioides simplex VKM Ac-2033D to phytosterol and cortisone 21-acetate.BMC biotechnology · 2021Article
- Experimental and Genomic Evaluation of the Oestrogen Degrading BacteriumFrontiers in microbiology · 2021Article
- Article
- Review
- New insights into the genome of Rhodococcus ruber strain Chol-4.BMC genomics · 2019Article
- Metabolic engineering of Rhodococcus ruber Chol-4: A cell factory for testosterone production.PloS one · 2019Article
- Degradation of crude oil by mixed cultures of bacteria isolated from the Qinghai-Tibet plateau and comparative analysis of metabolic mechanisms.Environmental science and pollution research international · 2019Article
- Identification, function, and application of 3-ketosteroid Δ1-dehydrogenase isozymes in Mycobacterium neoaurum DSM 1381 for the production of steroidic synthons.Microbial cell factories · 2018Article
- New Insights on Steroid Biotechnology.Frontiers in microbiology · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Rhodococcus ruber strain Chol-4 genome contains at least three putative 3-ketosteroid Δ
resultsThe three R. ruber KstD enzymes have different substrate profiles. KstD1 shows preference for 9OHAD and testosterone, followed by progesterone, deoxy corticosterone AD and, finally, 4-BNC, corticosterone and 19OHAD. KstD2 shows maximum preference for progesterone followed by 5α-Tes, DOC, AD testosterone, 4-BNC and lastly 19OHAD, corticosterone and 9OHAD. KstD3 preference is for saturated steroid substrates (5α-Tes) followed by progesterone and DOC. A preliminary attempt to model the catalytic pocket of the KstD proteins revealed some structural differences probably related to their catalytic differences. The expression of kstD genes has been studied by RT-PCR and RT-qPCR. All the kstD genes are transcribed under all the conditions assayed, although an additional induction in cholesterol and AD could be observed for kstD1 and in cholesterol for kstD3. Co-transcription of some correlative genes could be stated. The transcription initiation signals have been searched, both in silico and in vivo. Putative promoters in the intergenic regions upstream the kstD1, kstD2 and kstD3 genes were identified and probed in an apramycin-promoter-test vector, leading to the functional evidence of those R. ruber kstD promoters.
conclusionsAt least three putative 3-ketosteroid Δ
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.