ArticleBMC evolutionary biology2017
Duplication and concerted evolution of MiSp-encoding genes underlie the material properties of minor ampullate silks of cobweb weaving spiders.
Article in BMC evolutionary biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 38 citations in OpenAlex.
- Characteristic Evaluation of Recombinant MiSp/Poly(lactic-International journal of molecular sciences · 2023Article
- Chromosome-level genome and the identification of sex chromosomes in Uloborus diversus.GigaScience · 2022Article
- Rapid molecular diversification and homogenization of clustered major ampullate silk genes in Argiope garden spiders.PLoS genetics · 2022Article
- Chromosome-level genome assembly of the black widow spider Latrodectus elegans illuminates composition and evolution of venom and silk proteins.GigaScience · 2022Article
- Recombinant Spider Silk: Promises and Bottlenecks.Frontiers in bioengineering and biotechnology · 2022Review
- The Tetragnatha kauaiensis Genome Sheds Light on the Origins of Genomic Novelty in Spiders.Genome biology and evolution · 2021Article
- Hybrid Spider Silk with Inorganic Nanomaterials.Nanomaterials (Basel, Switzerland) · 2020Review
- Expanding Canonical Spider Silk Properties through a DNA Combinatorial Approach.Materials (Basel, Switzerland) · 2020Article
- Structural Diversity of Native Major Ampullate, Minor Ampullate, Cylindriform, and Flagelliform Silk Proteins in Solution.Biomacromolecules · 2020Article
- The common house spider, Parasteatoda tepidariorum, maintains silk gene expression on sub-optimal diet.PloS one · 2020Article
- Toward Spider Glue: Long Read Scaffolding for Extreme Length and Repetitious Silk Family Genes AgSp1 and AgSp2 with Insights into Functional Adaptation.G3 (Bethesda, Md.) · 2019Article
- The transcriptome of Darwin's bark spider silk glands predicts proteins contributing to dragline silk toughness.Communications biology · 2019Article
- Egg Case Silk Gene Sequences fromG3 (Bethesda, Md.) · 2018Article
- Silk genes and silk gene expression in the spider Tengella perfuga (Zoropsidae), including a potential cribellar spidroin (CrSp).PloS one · 2018Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundOrb-web weaving spiders and their relatives use multiple types of task-specific silks. The majority of spider silk studies have focused on the ultra-tough dragline silk synthesized in major ampullate glands, but other silk types have impressive material properties. For instance, minor ampullate silks of orb-web weaving spiders are as tough as draglines, due to their higher extensibility despite lower strength. Differences in material properties between silk types result from differences in their component proteins, particularly members of the spidroin (spider fibroin) gene family. However, the extent to which variation in material properties within a single silk type can be explained by variation in spidroin sequences is unknown. Here, we compare the minor ampullate spidroins (MiSp) of orb-weavers and cobweb weavers. Orb-web weavers use minor ampullate silk to form the auxiliary spiral of the orb-web while cobweb weavers use it to wrap prey, suggesting that selection pressures on minor ampullate spidroins (MiSp) may differ between the two groups.
resultsWe report complete or nearly complete MiSp sequences from five cobweb weaving spider species and measure material properties of minor ampullate silks in a subset of these species. We also compare MiSp sequences and silk properties of our cobweb weavers to published data for orb-web weavers. We demonstrate that all our cobweb weavers possess multiple MiSp loci and that one locus is more highly expressed in at least two species. We also find that the proportion of β-spiral-forming amino acid motifs in MiSp positively correlates with minor ampullate silk extensibility across orb-web and cobweb weavers.
conclusionsMiSp sequences vary dramatically within and among spider species, and have likely been subject to multiple rounds of gene duplication and concerted evolution, which have contributed to the diverse material properties of minor ampullate silks. Our sequences also provide templates for recombinant silk proteins with tailored properties.
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