ArticleTissue engineering. Part A2017
Co-Seeding Human Endothelial Cells with Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells on Calcium Phosphate Scaffold Enhances Osteogenesis and Vascularization in Rats.
Article in Tissue engineering. Part A, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- A 3D prevascularized calcium phosphate cement scaffold for accelerated alveolar bone regeneration and angiogenesis in rats.BMC oral health · 2026Article
- Stromal vascular fraction self-assembles vascularized osteogenic organoids with immunomodulatory functions.Bioactive materials · 2026Article
- Preclinical Evaluation and Advancements in Vascularized Bone Tissue Engineering.Biomimetics (Basel, Switzerland) · 2025Review
- Induced Pluripotent (iPSC) and Mesenchymal (MSC) Stem Cells for In Vitro Disease Modeling and Regenerative Medicine.International journal of molecular sciences · 2025Review
- Magnetic Nanocomposite Hydrogels in Orthopedics: Applications and Perspectives.International journal of nanomedicine · 2025Review
- Investigation of the biological activity of hydroxyapatite on vascular endothelial cells.Heliyon · 2023Article
- Potential and Limitations of Induced Pluripotent Stem Cells-Derived Mesenchymal Stem Cells in Musculoskeletal Disorders Treatment.Biomolecules · 2023Review
- Advances in Animal Models for Studying Bone Fracture Healing.Bioengineering (Basel, Switzerland) · 2023Review
- Dental Pulp Stem Cells for Bone Tissue Engineering: A Literature Review.Stem cells international · 2023Review
- Article
- Oral mucosa equivalents, prevascularization approaches, and potential applications.Connective tissue research · 2022Review
- Chitosan/hydroxyapatite nanocomposite scaffolds to modulate osteogenic and inflammatory response.Journal of biomedical materials research. Part A · 2022Article
- Biodegradable Poly-ε-Caprolactone Scaffolds with ECFCs and iMSCs for Tissue-Engineered Heart Valves.International journal of molecular sciences · 2022Article
- Synthetic materials in craniofacial regenerative medicine: A comprehensive overview.Frontiers in bioengineering and biotechnology · 2022Review
- Periodontal ligament stem cell-based bioactive constructs for bone tissue engineering.Frontiers in bioengineering and biotechnology · 2022Review
- Human Periodontal Ligament Stem Cell and Umbilical Vein Endothelial Cell Co-Culture to Prevascularize Scaffolds for Angiogenic and Osteogenic Tissue Engineering.International journal of molecular sciences · 2021Article
- A novel device for treatment of osteonecrosis of femoral head: Feasibility and preliminary efficacy of animal study.Journal of orthopaedic translation · 2021Article
- Stem Cells and Their Derivatives-Implications for Alveolar Bone Regeneration: A Comprehensive Review.International journal of molecular sciences · 2021Review
- In Vivo Evaluation of Decellularized Human Tooth Scaffold for Dental Tissue Regeneration.Applied sciences (Basel, Switzerland) · 2021Article
- Vascularization strategies in tissue engineering approaches for soft tissue repair.Journal of tissue engineering and regenerative medicine · 2021Review
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10 authors.
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Abstract
A major challenge in repairing large bone defects with tissue-engineered constructs is the poor vascularization in the defect. The lack of vascular networks leads to insufficient oxygen and nutrients supply, which compromises the survival of seeded cells. To achieve favorable regenerative effects, prevascularization of tissue-engineered constructs by co-culturing of endothelial cells and bone cells is a promising strategy. The aim of this study was to investigate the effects of human-induced pluripotent stem cell-derived mesenchymal stem cells (hiPSC-MSCs) co-cultured with human umbilical vein endothelial cells (HUVECs) for prevascularization of calcium phosphate cement (CPC) scaffold on bone regeneration in vivo for the first time. HUVECs co-cultured with hiPSC-MSCs formed microcapillary-like structures in vitro. HUVECs promoted mineralization of hiPSC-MSCs on CPC scaffolds. Four groups were tested in a cranial bone defect model in nude rats: (1) CPC scaffold alone (CPC control); (2) HUVEC-seeded CPC (CPC-HUVEC); (3) hiPSC-MSC-seeded CPC (CPC-hiPSC-MSC); and (4) HUVECs co-cultured with hiPSC-MSCs on CPC scaffolds (co-culture group). After 12 weeks, the co-culture group achieved the greatest new bone area percentage of 46.38% ± 3.8% among all groups (p < 0.05), which was more than four folds of the 10.61% ± 1.43% of CPC control. In conclusion, HUVECs co-cultured with hiPSC-MSCs substantially promoted bone regeneration. The novel construct of HUVECs co-cultured with hiPSC-MSCs delivered via CPC scaffolds is promising to enhance bone and vascular regeneration in orthopedic applications.
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