ArticleAmerican journal of respiratory cell and molecular biology2017
Efficiency and Specificity of Gene Deletion in Lung Epithelial Doxycycline-Inducible Cre Mice.
Article in American journal of respiratory cell and molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- An ErbB2 splice variant lacking exon 16 drives lung carcinoma.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Phenotypic Diversity Caused by Differential Expression ofAmerican journal of respiratory cell and molecular biology · 2020Article
- Respiratory Enterovirus (like Parainfluenza Virus) Can Cause Chronic Lung Disease if Protection by Airway Epithelial STAT1 Is Lost.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Depletion of Nesprin-2 is associated with an embryonic lethal phenotype in mice.Nucleus (Austin, Tex.) · 2018Article
- The signaling adaptor insulin receptor substrate 2 (IRS2) limits lethal host responses induced by influenza infection.Innate immunityArticle
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The transgenic mouse strains surfactant protein C-reverse tetracycline transactivator (SP-C-rtTA), club cell secretory protein (CCSP)-rtTA, and tetracycline operator (TetO)-Cre have been invaluable for spatiotemporally regulating gene deletion in the pulmonary epithelium. In this study, we measured the efficiency and specificity of gene deletion that can be achieved in these mice using the Rosa26-eYFP reporter. Triple-transgenic mice (tTg or rtTA/TetO-Cre/Rosa-eYFP) were bred and treated with various doxycycline (dox) regimens to induce gene deletion, which was then quantified in various cell populations by flow cytometry. In these crosses, we found that the TetO-Cre transgene must be transmitted through the female parent to avoid germline gene deletion. With dox exposure during lung development, SP-C-tTg mice deleted in ∼65-75% of alveolar epithelial type II (ATII) cells, but in only ∼45-50% of the integrin β4
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Registered trials
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