ArticleJournal of visualized experiments : JoVE2017
A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- High throughput interrogation of human liver stellate cells reveals microenvironmental regulation of phenotype.Acta biomaterialia · 2022Pooled it
- Engineered microtissue systems for identifying the roles of Wnt and YAP signaling in hepatoblast differentiation and organization.Materials today. Bio · 2026Article
- Effect of 2D and 3D ECM and Biomechanical Cues on Human iPSC-Derived Liver Progenitor Cell Differentiation.Advanced healthcare materials · 2026Article
- Measurement of cellular traction forces during confined migration.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Matrix Tropism Influences Endometriotic Cell Attachment Patterns.Advanced functional materials · 2025Article
- Cell Chirality of Micropatterned Endometrial Microvascular Endothelial Cells.Advanced healthcare materials · 2024Article
- Effect of distinct ECM microenvironments on the genome-wide chromatin accessibility and gene expression responses of hepatic stellate cells.Acta biomaterialia · 2023Article
- The extracellular matrix mechanics in the vasculature.Nature cardiovascular research · 2023Review
- Engineered matrix microenvironments reveal the heterogeneity of liver sinusoidal endothelial cell phenotypic responses.APL bioengineering · 2022Article
- Modulation of human iPSC-derived hepatocyte phenotype via extracellular matrix microarrays.Acta biomaterialia · 2022Article
- Delineating cooperative effects of Notch and biomechanical signals on patterned liver differentiation.Communications biology · 2022Article
- Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.ACS biomaterials science & engineering · 2022Article
- Identification of the Differentiation Stages of Living Cells from the Six Most Immature Murine Hematopoietic Cell Populations by Multivariate Analysis of Single-Cell Raman Spectra.Analytical chemistry · 2022Article
- A primer to traction force microscopy.The Journal of biological chemistry · 2022Review
- Systems of conductive skin for power transfer in clinical applications.European biophysics journal : EBJ · 2022Review
- Elucidating Extracellular Matrix and Stiffness Control of Primary Human Hepatocyte Phenotype Via Cell Microarrays.Advanced materials interfaces · 2021Article
- High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology.Chemical reviews · 2021Review
- Development of an inexpensive Raman-compatible substrate for the construction of a microarray screening platform.The Analyst · 2020Article
- Mechanical Characterization for Cellular Mechanobiology: Current Trends and Future Prospects.Frontiers in bioengineering and biotechnology · 2020Review
- High Throughput Traction Force Microscopy for Multicellular Islands on Combinatorial Microarrays.Bio-protocol · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Microfabricated cellular microarrays, which consist of contact-printed combinations of biomolecules on an elastic hydrogel surface, provide a tightly controlled, high-throughput engineered system for measuring the impact of arrayed biochemical signals on cell differentiation. Recent efforts using cell microarrays have demonstrated their utility for combinatorial studies in which many microenvironmental factors are presented in parallel. However, these efforts have focused primarily on investigating the effects of biochemical cues on cell responses. Here, we present a cell microarray platform with tunable material properties for evaluating both cell differentiation by immunofluorescence and biomechanical cell-substrate interactions by traction force microscopy. To do so, we have developed two different formats utilizing polyacrylamide hydrogels of varying Young's modulus fabricated on either microscope slides or glass-bottom Petri dishes. We provide best practices and troubleshooting for the fabrication of microarrays on these hydrogel substrates, the subsequent cell culture on microarrays, and the acquisition of data. This platform is well-suited for use in investigations of biological processes for which both biochemical (e.g., extracellular matrix composition) and biophysical (e.g., substrate stiffness) cues may play significant, intersecting roles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.