ArticleScientific reports2017
Hyaluronan synthesis by developing cortical neurons in vitro.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- Epigenetic Regulation of Hyaluronan-Associated Genes in the Brain: Identifying Key Regulatory Sites.Epigenomes · 2026Review
- Hyaluronic acid: emerging roles and biomaterial innovations in Alzheimer's and Parkinson's disease therapy.Frontiers in pharmacology · 2026Review
- Glucocorticoids Modulate Expression of Perineuronal Net Component Genes and Parvalbumin During Development of Mouse Cortical Neurons.Molecular neurobiology · 2025Article
- Review
- Dynamic Organization of Neuronal Extracellular Matrix Revealed by HaloTag-HAPLN1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Derivatization of Hyaluronan to Target Neuroblastoma and Neuroglioma Expressing CD44.Pharmaceutics · 2024Article
- Novel extracellular matrix architecture on excitatory neurons revealed by HaloTag-HAPLN1.bioRxiv : the preprint server for biology · 2024Article
- Ischemic Preconditioning Alleviates Cerebral Ischemia-Reperfusion Injury by Interfering With Glycocalyx.Translational stroke research · 2023Article
- Perineuronal nets and the neuronal extracellular matrix can be imaged by genetically encoded labeling of HAPLN1bioRxiv : the preprint server for biology · 2023Article
- LongitudinalNeurophotonics · 2023Article
- Enzymatic Degradation of Cortical Perineuronal Nets Reverses GABAergic Interneuron Maturation.Molecular neurobiology · 2022Article
- An alginate-based encapsulation system for delivery of therapeutic cells to the CNS.RSC advances · 2022Article
- Matters of size: Roles of hyaluronan in CNS aging and disease.Ageing research reviews · 2021Review
- Microglia as hackers of the matrix: sculpting synapses and the extracellular space.Cellular & molecular immunology · 2021Review
- Synaptic Hyaluronan Synthesis and CD44-Mediated Signaling Coordinate Neural Circuit Development.Cells · 2021Article
- Review
- BDNF and JNK Signaling Modulate Cortical Interneuron and Perineuronal Net Development: Implications for Schizophrenia-Linked 16p11.2 Duplication Syndrome.Schizophrenia bulletin · 2021Article
- An Extracellular Perspective on CNS Maturation: Perineuronal Nets and the Control of Plasticity.International journal of molecular sciences · 2021Review
- Extracellular matrixes and neuroinflammation.BMB reports · 2020Review
- Hyaluronan regulates synapse formation and function in developing neural networks.Scientific reports · 2020Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hyaluronan is a linear glycosaminoglycan that forms the backbone of perineuronal nets around neurons in the cerebral cortex. However, it remains controversial whether neurons are capable of independent hyaluronan synthesis. Herein, we examined the expression of hyaluronan and hyaluronan synthases (HASs) throughout cortical neuron development in vitro. Enriched cultures of cortical neurons were established from E16 rats. Neurons were collected at days in vitro (DIV) 0 (4 h), 1, 3, 7, 14, and 21 for qPCR or immunocytochemistry. In the relative absence of glia, neurons exhibited HAS1-3 mRNA at all time-points. By immunocytochemistry, puncta of HAS2-3 protein and hyaluronan were located on neuronal cell bodies, neurites, and lamellipodia/growth cones from as early as 4 h in culture. As neurons matured, hyaluronan was also detected on dendrites, filopodia, and axons, and around synapses. Percentages of hyaluronan-positive neurons increased with culture time to ~93% by DIV21, while only half of neurons at DIV21 expressed the perineuronal net marker Wisteria floribunda agglutinin. These data clearly demonstrate that neurons in vitro can independently synthesise hyaluronan throughout all maturational stages, and that hyaluronan production is not limited to neurons expressing perineuronal nets. The specific structural localisation of hyaluronan suggests potential roles in neuronal development and function.
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