ArticleScientific reports2017
Visual detection and differentiation of Classic Swine Fever Virus strains using nucleic acid sequence-based amplification (NASBA) and G-quadruplex DNAzyme assay.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.
- Pooled analysis of nuclear acid sequence-based amplification for rapid diagnosis of Mycoplasma pneumoniae infection.Journal of clinical laboratory analysis · 2019Pooled it
- Emergent Molecular Techniques Applied to the Detection of Porcine Viruses.Veterinary sciences · 2023Review
- Isothermal nucleic acid amplification and its uses in modern diagnostic technologies.3 Biotech · 2023Review
- Detection of Multiplex NASBA RNA Products Using Colorimetric Split G Quadruplex Probes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A CRISPR/Cas12a-based DNAzyme visualization system for rapid, non-electrically dependent detection ofEmerging microbes & infections · 2022Article
- Dual-Mode Gold Nanoparticle-Based Method for Early Detection ofInternational journal of molecular sciences · 2022Article
- Visual detection of porcine epidemic diarrhea virus by recombinase polymerase amplification combined with lateral flow dipstrip.BMC veterinary research · 2022Article
- Isothermal amplification-based assays for rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2: Opportunities and recent developments.Reviews in medical virology · 2022Review
- The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens.Plants (Basel, Switzerland) · 2021Review
- Application of Nanoscale Materials and Nanotechnology Against Viral Infection: A Special Focus on Coronaviruses.Advances in experimental medicine and biology · 2021Article
- Sensing the classical swine fever virus with molecularly imprinted polymer on quartz crystal microbalance.Heliyon · 2020Article
- Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis.Biochemistry. Biokhimiia · 2020Review
- Trends in MERS-CoV, SARS-CoV, and SARS-CoV-2 (COVID-19) Diagnosis Strategies: A Patent Review.Frontiers in public health · 2020Review
- Toward a Rational Approach to Design Split G-Quadruplex Probes.ACS chemical biology · 2019Article
- Label-Free Pathogen Detection by a Deoxyribozyme Cascade with Visual Signal Readout.Sensors and actuators. B, Chemical · 2019Article
- Alphanumerical Visual Display Made of DNA Logic Gates for Drug Susceptibility Testing of Pathogens.Chembiochem : a European journal of chemical biology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The split G-quadruplex DNAzyme has emerged as a valuable tool for visual DNA detection. Here, we successfully integrated colorimetric split G-quadruplex DNAzyme assay with nucleic acid sequence-based amplification to generate a novel detection approach, allowing visual and rapid detection for the RNA of Shimen and HCLV strains of Classic Swine Fever Virus (CSFV). CSFV is a RNA virus that causes a highly contagious disease in domestic pigs and wild boar. With this method, we were able to detect as little as 10 copies/ml of CSF viral RNA within 3 h in serum samples taken from the field. No interference was encountered in the amplification and detection of Classic Swine Fever Virus in the presence of non-target RNA or DNA. Moreover, Shimen and HCLV strains of Classic Swine Fever Virus could be easily differentiated using the NASBA-DNAzyme system. These findings indicate the NASBA-DNAzyme system is a rapid and practical technique for detecting and discriminating CSFV strains and may be applied to the detection of other RNA viruses.
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