ArticlePlant molecular biology2017
The molecular mechanism underlying anthocyanin metabolism in apple using the MdMYB16 and MdbHLH33 genes.
Article in Plant molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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96 citing papers in PubMed, 235 citations in OpenAlex.
- BrANL2 negatively regulates the anthocyanin biosynthesis by depressing BrPAL, BrC4H, and Br4CL in purple flowering Chinese cabbage (Brassica rapa var. parachinensis).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Evolutionary and functional divergence of MYB regulatory networks in flavonoid biosynthesis across Poaceae, non-Poaceae monocots, and eudicot horticultural plants.Horticulture research · 2026Review
- Molecular Mechanisms and Regulation of Anthocyanin Biosynthesis in Rosaceae Fruits: A Review.Food science & nutrition · 2026Review
- R2R3-MYB transcription factor MYB113 specifically regulates anthocyanin accumulation in Lycium ruthenicum.Plant cell reports · 2026Article
- Ethylene-responsive CmERF3-CmMYB4 cascade suppresses anthocyanin biosynthesis inHorticulture research · 2026Article
- Integrated Metabolome and Transcriptome Analysis Reveals the Effect of Anthocyanins on Flower Color Variation inBiology · 2026Article
- Transcription factor MdMYB6L represses anthocyanin biosynthesis of apple skin at low-altitude regions.Plant cell reports · 2026Article
- Genome-Wide Identification of the MYB Family inGenes · 2026Article
- LlR3MYB-mediated flavonoid biosynthesis confers cold stress tolerance inHorticulture research · 2026Article
- The telomere-to-telomere haplotype genome provides in-depth insights into the molecular mechanisms of the anthocyanin deficiency phenotype in Prunus mume.Molecular horticulture · 2026Article
- Overexpression of a Tonoplast Malate Transporter Gene Leads to Enhanced Anthocyanin Biosynthesis in Apple.Plant biotechnology journal · 2026Article
- Molecular insights into the regulation of flavonoid biosynthesis in fruits.Horticulture research · 2026Article
- Whole genome identification and operational analysis of theFrontiers in plant science · 2026Article
- Molecular Interaction of Genes Related to Anthocyanin, Lipid and Wax Biosynthesis in Apple Red-Fleshed Fruits.International journal of molecular sciences · 2025Article
- Review
- The R3-MYB Transcription Factor DcMYB56 Regulates Anthocyanin Accumulation by Activating the Expression of Anthocyanin Biosynthesis-Related Genes inPlants (Basel, Switzerland) · 2025Article
- Characterization of a KANADI-like transcription factor that suppresses pear anthocyanin biosynthesis.Horticulture research · 2025Article
- Ethylene promotes anthocyanin synthesis in 'Viviana' lily via the LvMYB5-LvERF113-LvMYB1 module.Horticulture research · 2025Article
- Phosphorylation of the strawberry MADS-box CMB1 regulates ripening via the catabolism of abscisic acid.The New phytologist · 2025Article
- Transcription FactorPlants (Basel, Switzerland) · 2025Article
36 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
key messageMdMYB16 forms homodimers and directly inhibits anthocyanin synthesis via its C-terminal EAR repressor. It weakened the inhibitory effect of MdMYB16 on anthocyanin synthesis when overexpressing MdbHLH33 in callus overexpressing MdMYB16. MdMYB16 could interact with MdbHLH33. Anthocyanins are strong antioxidants that play a key role in the prevention of cardiovascular disease, cancer, and diabetes. The germplasm of Malus sieversii f. neidzwetzkyana is important for the study of anthocyanin metabolism. To date, only limited studies have examined the negative regulatory mechanisms underlying anthocyanin synthesis in apple. Here, we analyzed the relationship between anthocyanin levels and MdMYB16 expression in mature Red Crisp 1-5 apple (M. domestica) fruit, generated an evolutionary tree, and identified an EAR suppression sequence and a bHLH binding motif of the MdMYB16 protein using protein sequence analyses. Overexpression of MdMYB16 or MdMYB16 without bHLH binding sequence (LBSMdMYB16) in red-fleshed callus inhibited MdUFGT and MdANS expression and anthocyanin synthesis. However, overexpression of MdMYB16 without the EAR sequence (LESMdMYB16) in red-fleshed callus had no inhibitory effect on anthocyanin. The yeast one-hybrid assay showed that MdMYB16 and LESMdMYB16 interacted the promoters of MdANS and MdUFGT, respectively. Yeast two-hybrid, pull-down, and bimolecular fluorescence complementation assays showed that MdMYB16 formed homodimers and interacted with MdbHLH33, however, the LBSMdMYB16 could not interact with MdbHLH33. We overexpressed MdbHLH33 in callus overexpressing MdMYB16 and found that it weakened the inhibitory effect of MdMYB16 on anthocyanin synthesis. Together, these results suggested that MdMYB16 and MdbHLH33 may be important part of the regulatory network controlling the anthocyanin biosynthetic pathway.
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