ArticlePlasmid2017
Ordering the mob: Insights into replicon and MOB typing schemes from analysis of a curated dataset of publicly available plasmids.
Article in Plasmid, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 79 citations in OpenAlex.
- Plasmids serve as vehicles and reservoirs of type VI secretion systems.ISME communications · 2026Article
- Challenges and considerations for whole-genome-based antimicrobial resistance plasmid investigations.Antimicrobial agents and chemotherapy · 2025Review
- Bacterial warfare is associated with virulence and antimicrobial resistance.Nature communications · 2025Article
- Plasmid diversity ofMicrobiology spectrum · 2025Article
- Towards quantifying plasmid similarity.Microbial genomics · 2024Review
- The plasmidome associated with Gram-negative bloodstream infections: A large-scale observational study using complete plasmid assemblies.Nature communications · 2024Article
- Phenotypic and Molecular Characterization of Hypervirulent and Multidrug-ResistantThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2024Article
- Structure and dispersion of the conjugative mobilome in surface ocean bacterioplankton.ISME communications · 2024Article
- Systematic analysis of plasmids of theMicrobial genomics · 2023Article
- Mge-cluster: a reference-free approach for typing bacterial plasmids.NAR genomics and bioinformatics · 2023Article
- Conjugative Plasmid pPPUT-Tik1-1 from a PermafrostInternational journal of molecular sciences · 2023Article
- A global survey ofMicrobial genomics · 2023Article
- Article
- Factors associated with plasmid antibiotic resistance gene carriage revealed using large-scale multivariable analysis.Scientific reports · 2023Article
- Vertical and horizontal gene transfer tradeoffs direct plasmid fitness.Molecular systems biology · 2023Article
- Plasmid Composition, Antimicrobial Resistance and Virulence Genes Profiles of Ciprofloxacin- and Third-Generation Cephalosporin-Resistant FoodborneMicroorganisms · 2023Article
- Development of an antimicrobial resistance plasmid transfer gene database for enteric bacteria.Frontiers in bioinformatics · 2023Article
- Elucidation of the Bovine Intramammary Bacteriome and Resistome from healthy cows of Swiss dairy farms in the Canton Tessin.Frontiers in microbiology · 2023Article
- Long-Read Whole Genome Sequencing Elucidates the Mechanisms of Amikacin Resistance in Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2022Article
- mobileOG-db: a Manually Curated Database of Protein Families Mediating the Life Cycle of Bacterial Mobile Genetic Elements.Applied and environmental microbiology · 2022Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Plasmid typing can provide insights into the epidemiology and transmission of plasmid-mediated antibiotic resistance. The principal plasmid typing schemes are replicon typing and MOB typing, which utilize variation in replication loci and relaxase proteins respectively. Previous studies investigating the proportion of plasmids assigned a type by these schemes ('typeability') have yielded conflicting results; moreover, thousands of plasmid sequences have been added to NCBI in recent years, without consistent annotation to indicate which sequences represent complete plasmids. Here, a curated dataset of complete Enterobacteriaceae plasmids from NCBI was compiled, and used to assess the typeability and concordance of in silico replicon and MOB typing schemes. Concordance was assessed at hierarchical replicon type resolutions, from replicon family-level to plasmid multilocus sequence type (pMLST)-level, where available. We found that 85% and 65% of the curated plasmids could be replicon and MOB typed, respectively. Overall, plasmid size and the number of resistance genes were significant independent predictors of replicon and MOB typing success. We found some degree of non-concordance between replicon families and MOB types, which was only partly resolved when partitioning plasmids into finer-resolution groups (replicon and pMLST types). In some cases, non-concordance was attributed to ambiguous boundaries between MOBP and MOBQ types; in other cases, backbone mosaicism was considered a more plausible explanation. β-lactamase resistance genes tended not to show fidelity to a particular plasmid type, though some previously reported associations were supported. Overall, replicon and MOB typing schemes are likely to continue playing an important role in plasmid analysis, but their performance is constrained by the diverse and dynamic nature of plasmid genomes.
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Registered trials
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