Evidence map›Paper›PMID 28285985›Full record

Trial reportVaccine2017

Cryopreservation-related loss of antigen-specific IFNγ producing CD4

Tom Ford, Claire Wenden, Alison Mbekeani, Len Dally, Josephine H Cox, Merribeth Morin, Nicola Winstone, Adrian V S Hill, Jill Gilmour, Katie J Ewer

Open access · hybridAbstract readClinical Trial, Phase IClinical Trial, Phase IIMulticenter Study
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Vaccine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 56 citations in OpenAlex.

  1. Trial
  2. Article
  3. Current Role of CAR-T Therapy in Haematological Care.Advances in experimental medicine and biology · 2025
    Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. VIDASThe international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease · 2022
    Article
  15. CyTOFFrontiers in immunology · 2022
    Review
  16. Article
  17. Article
  18. Cryopreservation of NK and T Cells Without DMSO for Adoptive Cell-Based Immunotherapy.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Tom FordIAVI-HIL, Human Immunology Laboratory, International AIDS Vaccine Initiative, London, UK; Department of Medicine, Imperial College, London, UK. Electronic address: t.ford@imperial.ac.uk.
Claire WendenIAVI-HIL, Human Immunology Laboratory, International AIDS Vaccine Initiative, London, UK; Department of Medicine, Imperial College, London, UK.
Alison MbekeaniIAVI-HIL, Human Immunology Laboratory, International AIDS Vaccine Initiative, London, UK; Department of Medicine, Imperial College, London, UK.
Len DallyEMMES Corporation, Rockville, MD, USA.
Josephine H CoxIAVI-HIL, Human Immunology Laboratory, International AIDS Vaccine Initiative, London, UK.
Merribeth MorinPATH Malaria Vaccine Initiative, Washington, DC, USA.
Nicola WinstoneIAVI-HIL, Human Immunology Laboratory, International AIDS Vaccine Initiative, London, UK; Department of Medicine, Imperial College, London, UK.
Adrian V S HillCentre for Clinical Vaccinology and Tropical Medicine and the Jenner Institute Laboratories, University of Oxford, UK.
Jill GilmourIAVI-HIL, Human Immunology Laboratory, International AIDS Vaccine Initiative, London, UK; Department of Medicine, Imperial College, London, UK.
Katie J EwerCentre for Clinical Vaccinology and Tropical Medicine and the Jenner Institute Laboratories, University of Oxford, UK.
Imperial College London · GBInternational HIV/AIDS Alliance · GBUniversity of Oxford · GBEmmes (United States) · USU.S. President's Malaria Initiative · US

Funding

Wellcome Trust
6 · The paper itself

Abstract

Ex vivo functional immunoassays such as ELISpot and intracellular cytokine staining (ICS) by flow cytometry are crucial tools in vaccine development both in the identification of novel immunogenic targets and in the immunological assessment of samples from clinical trials. Cryopreservation and subsequent thawing of PBMCs via validated processes has become a mainstay of clinical trials due to processing restrictions inherent in the disparate location and capacity of trial centres, and also in the need to standardize biological assays at central testing facilities. Logistical and financial requirement to batch process samples from multiple study timepoints are also key. We used ELISpot and ICS assays to assess antigen-specific immunogenicity in blood samples taken from subjects enrolled in a phase II malaria heterologous prime-boost vaccine trial and showed that the freeze thaw process can result in a 3-5-fold reduction of malaria antigen-specific IFNγ-producing CD3

Indexed as

CryopreservationCD4-Positive T-LymphocytesClinical Trials, Phase II as TopicDrug Evaluation, PreclinicalEnzyme-Linked Immunospot AssayFlow CytometryHumansInterferon-gammaMalaria VaccinesSpecimen HandlingStaining and LabelingInterferon-gammaMalaria VaccinesChAd63CryopreservationCSPMalariaMVAPBMCTRAP

Identifiers

PMID28285985
PMCPMC5387668
OpenAlexW2592394892

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.