Evidence map›Paper›PMID 28285879›Full record

ArticleStem cell reports2017

Lineage Specification from Prostate Progenitor Cells Requires Gata3-Dependent Mitotic Spindle Orientation.

Maxwell E R Shafer, Alana H T Nguyen, Mathieu Tremblay, Sophie Viala, Mélanie Béland, Nicholas R Bertos, Morag Park, Maxime Bouchard

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Maxwell E R ShaferRosalind and Morris Goodman Cancer Research Centre, McGill University, 1160 Pine Avenue West, Room 415, Montreal, QC H3A 1A3, Canada; Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, QC H3G 1Y6, Canada.
Alana H T NguyenRosalind and Morris Goodman Cancer Research Centre, McGill University, 1160 Pine Avenue West, Room 415, Montreal, QC H3A 1A3, Canada.
Mathieu TremblayRosalind and Morris Goodman Cancer Research Centre, McGill University, 1160 Pine Avenue West, Room 415, Montreal, QC H3A 1A3, Canada; Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, QC H3G 1Y6, Canada.
Sophie VialaRosalind and Morris Goodman Cancer Research Centre, McGill University, 1160 Pine Avenue West, Room 415, Montreal, QC H3A 1A3, Canada; Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, QC H3G 1Y6, Canada.
Mélanie BélandRosalind and Morris Goodman Cancer Research Centre, McGill University, 1160 Pine Avenue West, Room 415, Montreal, QC H3A 1A3, Canada.
Nicholas R BertosRosalind and Morris Goodman Cancer Research Centre, McGill University, 1160 Pine Avenue West, Room 415, Montreal, QC H3A 1A3, Canada.
Morag ParkRosalind and Morris Goodman Cancer Research Centre, McGill University, 1160 Pine Avenue West, Room 415, Montreal, QC H3A 1A3, Canada; Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, QC H3G 1Y6, Canada; Departments of Medicine and Oncology, McGill University, Montreal, QC H4A 3T2, Canada.
Maxime BouchardRosalind and Morris Goodman Cancer Research Centre, McGill University, 1160 Pine Avenue West, Room 415, Montreal, QC H3A 1A3, Canada; Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, QC H3G 1Y6, Canada. Electronic address: maxime.bouchard@mcgill.ca.
McGill University · CAMcGill University Health Centre · CA

Funding

CIHR
6 · The paper itself

Abstract

During prostate development, basal and luminal cell lineages are generated through symmetric and asymmetric divisions of bipotent basal cells. However, the extent to which spindle orientation controls division symmetry or cell fate, and the upstream factors regulating this process, are still elusive. We report that GATA3 is expressed in both prostate basal progenitor and luminal cells and that loss of GATA3 leads to a mislocalization of PRKCZ, resulting in mitotic spindle randomization during progenitor cell division. Inherently proliferative intermediate progenitor cells accumulate, leading to an expansion of the luminal compartment. These defects ultimately result in a loss of tissue polarity and defective branching morphogenesis. We further show that disrupting the interaction between PRKCZ and PARD6B is sufficient to recapitulate the spindle and cell lineage phenotypes. Collectively, these results identify a critical role for GATA3 in prostate lineage specification, and further highlight the importance of regulating spindle orientation for hierarchical cell lineage organization.

Indexed as

AnimalsCell PolarityEpithelial CellsGATA3 Transcription FactorGene DeletionMaleMice, Inbred C57BLProstateProtein Kinase CProtein Kinase C zetaSpindle ApparatusStem CellsGata3 protein, mouseGATA3 Transcription FactorProtein Kinase CProtein Kinase C zetaatypical protein kinase Caurothiomalatecell polarityepithelial stratificationGATA3lineage specificationpar complexprostate developmentprostate progenitor cellsspindle orientation

Identifiers

PMID28285879
PMCPMC5390093
OpenAlexW2593978516

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.