ArticleParasites & vectors2017
Diagnostic performance of ELISA, IFAT and Western blot for the detection of anti-Leishmania infantum antibodies in cats using a Bayesian analysis without a gold standard.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 69 citations in OpenAlex.
- Looking Beyond Leishmania infantum: Serological and Molecular Investigation of Leishmania spp. in Domestic Dogs and Cats from a Visceral Leishmaniasis-Endemic Area in Northeastern Brazil.Acta parasitologica · 2026Article
- LeishVet update and current recommendations on feline leishmaniosis.Parasites & vectors · 2026Review
- AB Blood System Phenotypes Are Not Associated withPathogens (Basel, Switzerland) · 2026Article
- Acute Phase Proteins in Cats Naturally Infected or Seropositive forAnimals : an open access journal from MDPI · 2026Article
- Diagnostic potential of a multi-antigen ELISA for feline leishmaniosis.Parasites & vectors · 2026Article
- Feline Leishmaniosis: A Retrospective Study of Seroprevalence in Cats in the Campania Region, Southern Italy.Animals : an open access journal from MDPI · 2025Article
- Chemical Sensors and Biosensors for Point-of-Care Testing of Pets: Opportunities for Individualized Diagnostics of Companion Animals.ACS sensors · 2025Review
- EAVLD 2024 - 7Italian journal of food safety · 2024Article
- Feline leishmaniosis in the Mediterranean Basin: a multicenter study.Parasites & vectors · 2024Article
- The erythrocyte sedimentation rate and other markers of inflammation in cats tested for Leishmania infantum and feline immunodeficiency virus antibodies.Parasites & vectors · 2024Article
- Feline immunodeficiency virus, feline leukemia virus and Leishmania spp. prevalence in cats from shelters in Mato Grosso do Sul, Brazil.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2024Article
- Immunoblotting Identification of Diagnostic Antigens ofTropical medicine and infectious disease · 2023Article
- Is leishmaniasis the new emerging zoonosis in the world?Veterinary research communications · 2023Review
- Article
- Tuning the Envelope Structure of Enzyme Nanoreactors for In Vivo Detoxification of Organophosphates.International journal of molecular sciences · 2023Article
- Comparative evaluation of some techniques used for the detection of rabies virus.Open veterinary journal · 2023Article
- Long-term follow-up of a case of feline leishmaniosis treated with a combination of allopurinol and meglumine antimoniate.The Canadian veterinary journal = La revue veterinaire canadienne · 2023Article
- Feline leishmaniosis: hematological and biochemical analysis.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2023Article
- Serological and Molecular Survey ofMicroorganisms · 2022Article
- Hemogram Findings in Cats from an Area Endemic forVeterinary sciences · 2022Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAnti-Leishmania antibodies are increasingly investigated in cats for epidemiological studies or for the diagnosis of clinical feline leishmaniosis. The immunofluorescent antibody test (IFAT), the enzyme-linked immunosorbent assay (ELISA) and western blot (WB) are the serological tests more frequently used. The aim of the present study was to assess diagnostic performance of IFAT, ELISA and WB to detect anti-L. infantum antibodies in feline serum samples obtained from endemic (n = 76) and non-endemic (n = 64) areas and from cats affected by feline leishmaniosis (n = 21) by a Bayesian approach without a gold standard.
methodsCut-offs were set at 80 titre for IFAT and 40 ELISA units for ELISA. WB was considered positive in presence of at least a 18 KDa band. Statistical analysis was performed through a written routine with MATLAB software in the Bayesian framework. The latent data and observations from the joint posterior were simulated in the Bayesian approach by an iterative Markov Chain Monte Carlo technique using the Gibbs sampler for estimating sensitivity and specificity of the three tests.
resultsThe median seroprevalence in the sample used for evaluating the performance of tests was estimated at 0.27 [credible interval (CI) = 0.20-0.34]. The median sensitivity of the three different methods was 0.97 (CI: 0.86-1.00), 0.75 (CI: 0.61-0.87) and 0.70 (CI: 0.56-0.83) for WB, IFAT and ELISA, respectively. Median specificity reached 0.99 (CI: 0.96-1.00) with WB, 0.97 (CI: 0.93-0.99) with IFAT and 0.98 (CI: 0.94-1.00) with ELISA. IFAT was more sensitive than ELISA (75 vs 70%) for the detection of subclinical infection while ELISA was better for diagnosing clinical leishmaniosis when compared with IFAT (98 vs 97%).
conclusionsThe overall performance of all serological techniques was good and the most accurate test for anti-Leishmania antibody detection in feline serum samples was WB.
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