ArticlePlant cell reports2017
Novel 9-cis/all-trans β-carotene isomerases from plastidic oil bodies in Dunaliella bardawil catalyze the conversion of all-trans to 9-cis β-carotene.
Article in Plant cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 20 citations in OpenAlex.
- Catalytic mechanism for β-carotene isomerisation and substrate selectivity by the strigolactone biosynthetic enzymes D27 and D27like1.Scientific reports · 2026Article
- Strategies for the efficient biosynthesis of β-carotene through microbial fermentation.World journal of microbiology & biotechnology · 2024Review
- Isomerization of carotenoids in photosynthesis and metabolic adaptation.Biophysical reviews · 2023Review
- Human nutritional relevance and suggested nutritional guidelines for vitamin A5/X and provitamin A5/X.Nutrition & metabolism · 2023Review
- Roles of Two Phytoene Synthases and Orange Protein in Carotenoid Metabolism of the β-Carotene-AccumulatingMicrobiology spectrum · 2023Article
- A Comparison of β-Carotene, Phytoene and Amino Acids Production inFoods (Basel, Switzerland) · 2021Article
- Stereoisomers of Colourless Carotenoids from the Marine MicroalgaMolecules (Basel, Switzerland) · 2020Article
- Red Light Control of β-Carotene Isomerisation toAntioxidants (Basel, Switzerland) · 2019Article
- A hypothesis about the origin of carotenoid lipid droplets in the green algae Dunaliella and Haematococcus.Planta · 2019Review
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Authors and funding
2 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
key messageWe identified and demonstrated the function of 9-cis/all-trans β-carotene isomerases in plastidic globules of Dunaliella bardawil, the species accumulating the highest levels of 9-cis β-carotene that is essential for humans. The halotolerant alga Dunaliella bardawil is unique in that it accumulates under light stress high levels of β-carotene in plastidic lipid globules. The pigment is composed of two major isomers: all-trans β-carotene, the common natural form of this pigment, and 9-cis β-carotene. The biosynthetic pathway of β-carotene is known, but it is not clear how the 9-cis isomer is formed. We identified in plastidic lipid globules that were isolated from D. bardawil two proteins with high sequence homology to the D27 protein-a 9-cis/all-trans β-carotene isomerase from rice (Alder et al. Science 335:1348-1351, 2012). The proteins are enriched in the oil globules by 6- to 17-fold compared to chloroplast proteins. The expression of the corresponding genes, 9-cis-βC-iso1 and 9-cis-βC-iso2, is enhanced under light stress. The synthetic proteins catalyze in vitro conversion of all-trans to 9-cis β-carotene. Expression of the 9-cis-βC-iso1 or of 9-cis-βC-iso2 genes in an E. coli mutant line that harbors β-carotene biosynthesis genes enhanced the conversion of all-trans into 9-cis β-carotene. These results suggest that 9-cis-βC-ISO1 and 9-cis-βC-ISO2 proteins are responsible for the formation of 9-cis β-carotene in D. bardawil under stress conditions.
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