A semi-empirical glycosylation model of a camelid monoclonal antibody under hypothermia cell culture conditions.

Hengameh Aghamohseni, Maureen Spearman, Kaveh Ohadi, Katrin Braasch, Murray Moo-Young, Michael Butler, Hector M Budman

Abstract read

In one paragraph

Article in Journal of industrial microbiology & biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it

0cells of the map it votes in

3citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Hengameh AghamohseniFaculty of Engineering, Chemical Engineering Department, University of Waterloo, E6 Building, Room 3012, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada.

Maureen SpearmanMicrobiology Department, University of Manitoba, Winnipeg, MB, Canada.

Kaveh OhadiFaculty of Engineering, Chemical Engineering Department, University of Waterloo, E6 Building, Room 3012, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada.

Katrin BraaschMicrobiology Department, University of Manitoba, Winnipeg, MB, Canada.

Murray Moo-YoungFaculty of Engineering, Chemical Engineering Department, University of Waterloo, E6 Building, Room 3012, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada.

Michael ButlerMicrobiology Department, University of Manitoba, Winnipeg, MB, Canada.

Hector M BudmanFaculty of Engineering, Chemical Engineering Department, University of Waterloo, E6 Building, Room 3012, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada. hbudman@uwaterloo.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The impact of cell culture environment on the glycan distribution of a monoclonal antibody (mAb) has been investigated through a combination of experiments and modeling. A newly developed CHO DUXB cell line was cultivated at two levels of initial Glutamine (Gln) concentrations (0, 4 mM) and incubation temperatures of (33 and 37 °C) in batch operation mode. Hypothermia was applied either through the entire culture duration or only during the post-exponential phase. Beyond reducing cell growth and increasing productivity, hypothermia significantly altered the galactosylation index profiles as compared to control conditions. A novel semi-empirical dynamic model was proposed for elucidating the connections between the extracellular cell culture conditions to galactosylation index. The developed model is based on a simplified balance of nucleotides sugars and on the correlation between sugars' levels to the galactosylation index (GI). The model predictions were found to be in a good agreement with the experimental data. The proposed empirical model is expected to be useful for controlling the glycoprofiles by manipulating culture conditions.

Indexed as

Cold TemperatureAnimalsAntibodies, MonoclonalCamelidaeCell Culture TechniquesCell ProliferationCells, CulturedCHO CellsCricetulusGlutamineGlycosylationModels, BiologicalPolysaccharidesAntibodies, MonoclonalGlutaminePolysaccharidesChinese hamster ovary cellsGlycosylationHypothermiaMathematical modelingMonoclonal antibody

Identifiers

PMID28285402

DOI10.1007/s10295-017-1926-z

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.