ArticleFungal genetics and biology : FG & B2017
Genetic and biochemical characterization of the GH72 family of cell wall transglycosylases in Neurospora crassa.
Article in Fungal genetics and biology : FG & B, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
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- Evidencing New Roles for the Glycosyl-Transferase Cps1 in the Phytopathogenic FungusJournal of fungi (Basel, Switzerland) · 2022Article
- Differential Expression of Cell Wall Remodeling Genes Is Part of the Dynamic Phase-Specific Transcriptional Program of Conidial Germination ofJournal of fungi (Basel, Switzerland) · 2022Article
- Extracellular targeting of Neurospora crassa cell wall and secreted glycoproteins by DFG-5.Fungal genetics and biology : FG & B · 2022Article
- Emerging Roles of β-Glucanases in Plant Development and Adaptative Responses.Plants (Basel, Switzerland) · 2022Review
- From Cancer Therapy to Winemaking: The Molecular Structure and Applications of β-Glucans and β-1, 3-Glucanases.International journal of molecular sciences · 2022Review
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- Off the wall: The rhyme and reason ofCell surface (Amsterdam, Netherlands) · 2019Review
- The Genetics and Biochemistry of Cell Wall Structure and Synthesis inFrontiers in microbiology · 2019Review
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- What We Do Not Know about Fungal Cell Adhesion Molecules.Journal of fungi (Basel, Switzerland) · 2018Review
- The PHR Family: The Role of Extracellular Transglycosylases in Shaping Candida albicans Cells.Journal of fungi (Basel, Switzerland) · 2017Review
- Combined genome and transcriptome sequencing to investigate the plant cell wall degrading enzyme system in the thermophilic fungusBiotechnology for biofuels · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Neurospora crassa genome encodes five GH72 family transglycosylases, and four of these enzymes (GEL-1, GEL-2, GEL-3 and GEL-5) have been found to be present in the cell wall proteome. We carried out an extensive genetic analysis on the role of these four transglycosylases in cell wall biogenesis and demonstrated that the transglycosylases are required for the formation of a normal cell wall. As suggested by the proteomic analysis, we found that multiple transglycosylases were being expressed in N. crassa cells and that different combinations of the enzymes are required in different cell types. The combination of GEL-1, GEL-2 and GEL-5 is required for the growth of vegetative hyphae, while the GEL-1, GEL-2, GEL-3 combination is needed for the production of aerial hyphae and conidia. Our data demonstrates that the enzymes are redundant with partially overlapping enzymatic activities, which provides the fungus with a robust cell wall biosynthetic system. Characterization of the transglycosylase-deficient mutants demonstrated that the incorporation of cell wall proteins was severely compromised. Interestingly, we found that the transglycosylase-deficient mutant cell walls contained more β-1,3-glucan than the wild type cell wall. Our results demonstrate that the GH72 transglycosylases are not needed for the incorporation of β-1,3-glucan into the cell wall, but they are required for the incorporation of cell wall glycoprotein into the cell wall.
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