ArticleBMC infectious diseases2017
Increased sensitivity of NS1 ELISA by heat dissociation in acute dengue 4 cases.
Article in BMC infectious diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Engineered Nanobodies for early and accurate diagnosis of dengue virus infection.PLoS neglected tropical diseases · 2025Article
- Non-structural protein 1 and hematology parameters as predictors of dengue virus infection severity in Indonesia.Journal of medicine and life · 2023Article
- Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique.Tropical medicine and infectious disease · 2023Article
- Serological Diagnosis of Dengue.Methods in molecular biology (Clifton, N.J.) · 2022Article
- High performance dengue virus antigen-based serotyping-NS1-ELISA (plus): A simple alternative approach to identify dengue virus serotypes in acute dengue specimens.PLoS neglected tropical diseases · 2021Article
- Development of a novel NS1 competitive enzyme-linked immunosorbent assay for the early detection of Zika virus infection.PloS one · 2021Article
- Article
- Evaluation of novel rapid detection kits for dengue virus NS1 antigen in Dhaka, Bangladesh, in 2017.Virology journal · 2019Article
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7 authors.
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Abstract
backgroundDengue is an acute febrile illness considered the major arboviral disease in terms of morbidity, mortality, economic impact and dissemination worldwide. Brazil accounts for the highest notification rate, with circulation of all four dengue serotypes. The NS1 antigen is a dengue highly conserved specific soluble glycoprotein essential for viral replication and viability that can be detected 0 to 18 days from the onset of fever (peak first 3 days). It induces a strong humoral response and is known as a complement-fixing antigen. Lower NS1 test sensitivity occurs in secondary dengue infections probably due to immune complex formation impairing antigen detection by ELISA.
methodsWe compared the sensitivity of NS1 ELISA in heat dissociated and non-dissociated samples from 156 RT-PCR confirmed acute dengue-4 cases from 362 prospectively enrolled patients.
resultsSecondary infections accounted for 83.3% of cases. NS1 ELISA was positive in 42.5% and indeterminate in 10.2% of dengue-4 cases. After heat dissociation, 7 negative and 16 indeterminate samples turned positive, increasing the overall test sensitivity to 57.7%.
conclusionsAlthough it is time consuming and requires the use of specific laboratory equipment, NS1 ELISA combined with heat dissociation could be a slightly better alternative for triage in suspected dengue cases.
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