ArticleJournal of industrial microbiology & biotechnology2017
Reconstruction of amino acid biosynthetic pathways increases the productivity of 2-keto-L-gulonic acid in Ketogulonicigenium vulgare-Bacillus endophyticus consortium via genes screening.
Article in Journal of industrial microbiology & biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Microbial Interactions in a Vitamin C Industrial Fermentation System: Novel Insights and Perspectives.Applied and environmental microbiology · 2022Review
- Construction of synthetic microbial consortia for 2-keto-L-gulonic acid biosynthesis.Synthetic and systems biotechnology · 2022Article
- One-Step Biosynthesis of Vitamin C inFrontiers in microbiology · 2021Article
- Integrated proteomic and metabolomic analysis of a reconstructed three-species microbial consortium for one-step fermentation of 2-keto-L-gulonic acid, the precursor of vitamin C.Journal of industrial microbiology & biotechnology · 2019Article
- High-Throughput Screening of a 2-Keto-L-Gulonic Acid-ProducingFrontiers in bioengineering and biotechnology · 2019Article
- Establishing an innovative carbohydrate metabolic pathway for efficient production of 2-keto-L-gulonic acid in Ketogulonicigenium robustum initiated by intronic promoters.Microbial cell factories · 2018Article
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Authors and funding
7 authors at 1 institution in 1 country.
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Abstract
Defect in the amino acid biosynthetic pathways of Ketogulonicigenium vulgare, the producing strain for 2-keto-L-gulonic acid (2-KGA), is the key reason for its poor growth and low productivity. In this study, five different strains were firstly reconstructed by expressing absent genes in threonine, proline and histidine biosynthetic pathways for better 2-KGA productivity. When mono-cultured in the shake flasks, the strain SyBE_Kv02080002 expressing hsk from Gluconobacter oxydans in threonine biosynthetic pathway achieved the highest biomass and the titer increased by 25.13%. When co-cultured with Bacillus endophyticus, the fermentation cycle decreased by 28.57% than that of the original consortium in 5-L fermenter. Furthermore, reconstruction of threonine biosynthetic pathway resulted in up-regulation of genes encoding sorbosone dehydrogenase and idonate-dehydrogenase, which increased the 2-KGA productivity in SyBE_Kv02080002. This study shows that reconstruction of absent biosynthetic pathways in bacteria is an effective way to enhance the productivity of target products.
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