ArticlePlant molecular biology2017
Identification of alternatively spliced transcripts of rice phytochelatin synthase 2 gene OsPCS2 involved in mitigation of cadmium and arsenic stresses.
Article in Plant molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 123 citations in OpenAlex.
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- Amino Acids as Multifunctional Molecules in Plants: From Fundamental Metabolism to Precision Agriculture.Plants (Basel, Switzerland) · 2026Review
- Genome-Wide Identification of thePlants (Basel, Switzerland) · 2026Article
- Adaptive Plasticity of Phytochelatin Synthase Under Chromium Stress and Sulfur Availability inPlants (Basel, Switzerland) · 2026Article
- CiPCS regulated by CiMYB4 enhances the tolerance of Chrysanthemum indicum to cadmium stress.Plant cell reports · 2025Article
- Navigating the genetic basis of cadmium accumulation: a comprehensive analysis of low-cadmium accumulation rice.Molecular breeding : new strategies in plant improvement · 2025Article
- Overexpression of NTPCS1 Enhances Zn Tolerance in Tobacco.Plants (Basel, Switzerland) · 2025Article
- Phytochelatins and Cadmium Mitigation: Harnessing Genetic Avenues for Plant Functional Manipulation.International journal of molecular sciences · 2025Review
- Ancient duplication and functional differentiation of phytochelatin synthases is conserved in plant genomes.Horticulture research · 2025Article
- Advances in RNA Interference for Plant Functional Genomics: Unveiling Traits, Mechanisms, and Future Directions.Applied biochemistry and biotechnology · 2024Review
- Phytochelatin Synthase: An In Silico Comparative Analysis in Cyanobacteria and Eukaryotic Microalgae.Plants (Basel, Switzerland) · 2024Article
- CRISPR-Cas9 mediated understanding of plants' abiotic stress-responsive genes to combat changing climatic patterns.Functional & integrative genomics · 2024Review
- Candidate Genes and Favorable Haplotypes Associated with Iron Toxicity Tolerance in Rice.International journal of molecular sciences · 2024Article
- Rice Big Grain1 improves grain yield in ectopically expressing rice and heterologously expressing tobacco plants.Plant molecular biology · 2024Article
- Review
- Effects of different phosphorus fertilizers on cadmium absorption and accumulation in rice under low-phosphorus and rich-cadmium soil.Environmental science and pollution research international · 2024Article
- Regulatory Mechanisms Underlying Arsenic Uptake, Transport, and Detoxification in Rice.International journal of molecular sciences · 2023Review
- Negative Impacts of Arsenic on Plants and Mitigation Strategies.Plants (Basel, Switzerland) · 2023Review
- Phytochelatins: Sulfur-Containing Metal(loid)-Chelating Ligands in Plants.International journal of molecular sciences · 2023Review
- Genome-Scale Profiling and High-Throughput Analyses Unravel the Genetic Basis of Arsenic Content Variation in Rice.Frontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageThe OsPCS2 exhibits root- and shoot-specific differential ratios of alternatively spliced transcripts in indica rice under Cd stress, and plays role in Cd and As stress tolerance and accumulation. Enzymatic activity of phytochelatin synthase (PCS) in plant produces phytochelatins, which help in sequestration of heavy metal(loid)s inside the cell vacuole to alleviate toxicity. Here we report that among the two PCS genes-OsPCS1 and OsPCS2 in indica rice (Oryza sativa) cultivar, the OsPCS2 produces an alternatively spliced OsPCS2b transcript that bears the unusual premature termination codon besides the canonically spliced OsPCS2a transcript. Root- and shoot-specific differential ratios of alternatively spliced OsPCS2a and OsPCS2b transcript expressions were observed under cadmium stress. Saccharomyces cerevisiae cells transformed with OsPCS2a exhibited increased cadmium (Cd) and arsenic (As) tolerance and accumulation, unlike the OsPCS2b transformed yeast cells. An intron-containing hairpin RNA-mediated gene silencing was carried out in endosperm-specific manner for efficient down-regulation of OsPCS genes in rice grains. Analysis of the transgenic rice lines grown under metal(loid) stress revealed almost complete absence of both OsPCS1 and OsPCS2 transcripts in the developing seeds coupled with the significant reduction in the content of Cd (~51%) and As (~35%) in grains compared with the non-transgenic plant. Taken together, the findings indicate towards a crucial role played by the tissue-specific alternative splicing and relative abundance of the OsPCS2 gene during heavy metal(loid) stress mitigation in rice plant.
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