Evidence map›Paper›PMID 28283797›Full record

Trial reportGeroScience2017

Influence of Nrf2 activators on subcellular skeletal muscle protein and DNA synthesis rates after 6 weeks of milk protein feeding in older adults.

Adam R Konopka, Jaime L Laurin, Robert V Musci, Christopher A Wolff, Justin J Reid, Laurie M Biela, Qian Zhang, Fredrick F Peelor, Christopher L Melby, Karyn L Hamilton and 1 more

Open access · bronzeAbstract readRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in GeroScience, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
4.0field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 43 citations in OpenAlex.

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  12. Reductive stress in striated muscle cells.Cellular and molecular life sciences : CMLS · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Adam R KonopkaDepartment of Health and Exercise Science, Colorado State University, 110 Moby B Complex, Fort Collins, CO, 80523, USA. Adam.Konopka@colostate.edu.
Jaime L LaurinDepartment of Health and Exercise Science, Colorado State University, 110 Moby B Complex, Fort Collins, CO, 80523, USA.
Robert V MusciDepartment of Health and Exercise Science, Colorado State University, 110 Moby B Complex, Fort Collins, CO, 80523, USA.
Christopher A WolffDepartment of Health and Exercise Science, Colorado State University, 110 Moby B Complex, Fort Collins, CO, 80523, USA.
Justin J ReidDepartment of Health and Exercise Science, Colorado State University, 110 Moby B Complex, Fort Collins, CO, 80523, USA.
Laurie M BielaDepartment of Health and Exercise Science, Colorado State University, 110 Moby B Complex, Fort Collins, CO, 80523, USA.
Qian ZhangDepartment of Health and Exercise Science, Colorado State University, 110 Moby B Complex, Fort Collins, CO, 80523, USA.
Fredrick F PeelorDepartment of Health and Exercise Science, Colorado State University, 110 Moby B Complex, Fort Collins, CO, 80523, USA.
Christopher L MelbyDepartment of Health and Exercise Science, Colorado State University, 110 Moby B Complex, Fort Collins, CO, 80523, USA.
Karyn L HamiltonDepartment of Health and Exercise Science, Colorado State University, 110 Moby B Complex, Fort Collins, CO, 80523, USA.
Benjamin F MillerDepartment of Health and Exercise Science, Colorado State University, 110 Moby B Complex, Fort Collins, CO, 80523, USA.
Colorado State University · US

Funding

Colorado Clinical and Translational Sciences InstituteUL1TR001082 · NCATS · UNIVERSITY OF COLORADO DENVER · PI SOKOL, RONALD J. · 2013 to 2017
$48.0M
NCATS NIH HHS UL1 TR001082
6 · The paper itself

Abstract

In older adults, chronic oxidative and inflammatory stresses are associated with an impaired increase in skeletal muscle protein synthesis after acute anabolic stimuli. Conjugated linoleic acid (CLA) and Protandim have been shown to activate nuclear factor erythroid-derived 2-like 2 (Nrf2), a transcription factor for the antioxidant response element and anti-inflammatory pathways. This study tested the hypothesis that compared to a placebo control (CON), CLA and Protandim would increase skeletal muscle subcellular protein (myofibrillar, mitochondrial, cytoplasmic) and DNA synthesis in older adults after 6 weeks of milk protein feeding. CLA decreased oxidative stress and skeletal muscle oxidative damage with a trend to increase messenger RNA (mRNA) expression of a Nrf2 target, NAD(P)H dehydrogenase quinone 1 (NQO1). However, CLA did not influence other Nrf2 targets (heme oxygenase-1 (HO-1), glutathione peroxidase 1 (Gpx1)) or protein or DNA synthesis. Conversely, Protandim increased HO-1 protein content but not the mRNA expression of downstream Nrf2 targets, oxidative stress, or skeletal muscle oxidative damage. Rates of myofibrillar protein synthesis were maintained despite lower mitochondrial and cytoplasmic protein syntheses after Protandim versus CON. Similarly, DNA synthesis was non-significantly lower after Protandim compared to CON. After Protandim, the ratio of protein to DNA synthesis tended to be greater in the myofibrillar fraction and maintained in the mitochondrial and cytoplasmic fractions, emphasizing the importance of measuring both protein and DNA synthesis to gain insight into proteostasis. Overall, these data suggest that Protandim may enhance proteostatic mechanisms of skeletal muscle contractile proteins after 6 weeks of milk protein feeding in older adults.

Indexed as

AgedDouble-Blind MethodDrugs, Chinese HerbalFemaleHeme Oxygenase-1HumansLinoleic Acids, ConjugatedMaleMiddle AgedMilk ProteinsMuscle ProteinsMuscle, SkeletalNAD(P)H Dehydrogenase (Quinone)NF-E2-Related Factor 2Oxidative StressRNA, MessengerDrugs, Chinese HerbalHeme Oxygenase-1Linoleic Acids, ConjugatedMilk ProteinsMuscle ProteinsNAD(P)H Dehydrogenase (Quinone)NF-E2-Related Factor 2ProtandimRNA, MessengerAnabolic resistanceInflammationOxidative stressProteostasisSarcopenia

Identifiers

PMID28283797
PMCPMC5411371
OpenAlexW2591978620

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.