Evidence map›Paper›PMID 28283525›Full record

ArticleApplied and environmental microbiology2017

Molecular Viability Testing of UV-Inactivated Bacteria.

Kris M Weigel, Felicia K Nguyen, Moira R Kearney, John S Meschke, Gerard A Cangelosi

Open access · bronzeAbstract readEvaluation Study
In one paragraph

Article in Applied and environmental microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 36 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. ProbioticMicroorganisms · 2023
    Article
  5. Article
  6. Review
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Kris M WeigelDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, USA.
Felicia K NguyenDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, USA.
Moira R KearneyDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, USA.
John S MeschkeDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, USA.
Gerard A CangelosiDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, USA gcang@uw.edu.
University of Washington · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

PCR is effective in detecting bacterial DNA in samples, but it is unable to differentiate viable bacteria from inactivated cells or free DNA fragments. New PCR-based analytical strategies have been developed to address this limitation. Molecular viability testing (MVT) correlates bacterial viability with the ability to rapidly synthesize species-specific rRNA precursors (pre-rRNA) in response to brief nutritional stimulation. Previous studies demonstrated that MVT can assess bacterial inactivation by chlorine, serum, and low-temperature pasteurization. Here, we demonstrate that MVT can detect inactivation of

Indexed as

BacteriaMicrobial ViabilityPolymerase Chain ReactionUltraviolet Raysbacterial inactivationdisinfectiondrinking watermolecular viability testing (MVT)propidium monoazide (PMA)ratiometric pre-rRNA analysisUV irradiationviability PCR (vPCR)viable but nonculturable (VBNC)

Identifiers

PMID28283525
PMCPMC5411506
OpenAlexW2593312090

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.