Evidence map›Paper›PMID 28283003›Full record

ArticleDrug delivery2017

Bacterial magnetic particles improve testes-mediated transgene efficiency in mice.

Chao Wang, Guanghong Sun, Ye Wang, Nana Kong, Yafei Chi, Leilei Yang, Qiliang Xin, Zhen Teng, Xu Wang, Yujun Wen and 2 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Drug delivery, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Delivering CRISPR to the HIV-1 reservoirs.Frontiers in microbiology · 2024
    Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Chao Wanga State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
Guanghong Suna State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
Ye Wanga State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
Nana Konga State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
Yafei Chib School of Basic Medical Science, Capital Medical University , Beijing , China.
Leilei Yangb School of Basic Medical Science, Capital Medical University , Beijing , China.
Qiliang Xina State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
Zhen Tenga State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
Xu Wanga State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
Yujun Wend Ningxia Key Laboratory of Cerebrocranial Diseases, Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Ningxia Medical University , Yinchuan , China.
Ying Lia State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.
Guoliang Xiaa State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University , Beijing , China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nano-scaled materials have been proved to be ideal DNA carriers for transgene. Bacterial magnetic particles (BMPs) help to reduce the toxicity of polyethylenimine (PEI), an efficient gene-transferring agent, and assist tissue transgene ex vivo. Here, the effectiveness of the BMP-PEI complex-conjugated foreign DNAs (BPDs) in promoting testes-mediated gene transfer (TMGT) in mouse was compared with that of liposome-conjugated foreign DNAs. The results proved that through testes injection, the clusters of BPDs successfully reached the cytoplasm and the nuclear of spermatogenesis cell, and expressed in testes of transgene founder mice. Additionally, the ratio of founder mice obtained from BPDs (88%) is about 3 times higher than the control (25%) (p < 0.05). Interestingly, the motility of sperms recovered from epididymis of the founder mice from BPD group were significantly improved, as compared with the control (p < 0.01). Based on classic breeding, the ratio of transgene mice within the first filial was significantly higher in BPDs compared with the control (73.8% versus 11.6%, p < 0.05). TMGT in this study did not produce visible histological changes in the testis. In conclusion, nano-scaled BPDs could be an alternative strategy for efficiently producing transgene mice in vivo.

Indexed as

Gene Transfer TechniquesTransgenesAnimalsFounder EffectGene Expression RegulationGenotypeGreen Fluorescent ProteinsIminesLiposomesMagnetosomesMagnetospirillumMaleMiceMice, TransgenicPhenotypePolyethylenesGreen Fluorescent ProteinsIminesLiposomespoly(ethylene imine)Polyethylenesbacterial magnetic particleefficientmiceTestistransgene

Identifiers

PMID28283003
PMCPMC8241085

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.