ArticleNanoscale research letters2017
A Rapid Detection Method of Brucella with Quantum Dots and Magnetic Beads Conjugated with Different Polyclonal Antibodies.
Article in Nanoscale research letters, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 39 citations in OpenAlex.
- An Immunomagnetic Bead-Based Sandwich ELISA for the Detection ofAnimals : an open access journal from MDPI · 2026Article
- Polyvinylpyrrolidone-modified iron single-atom nanozymes: preparation and application to highly sensitive immunomagnetic bead assay for tetracycline detection.Mikrochimica acta · 2026Article
- Nanoparticle Targeting with Antibodies in the Central Nervous System.BME frontiers · 2023Review
- Rapid detection ofRSC advances · 2021Article
- A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis.BMC veterinary research · 2021Article
- A New Optical Fiber Probe-Based Quantum Dots Immunofluorescence Biosensors in the Detection ofFrontiers in cellular and infection microbiology · 2021Article
- Rapid detection of brucellosis using a quantum dot-based immunochromatographic test strip.PLoS neglected tropical diseases · 2020Article
- Development of a DNA aptamer to detectIranian journal of veterinary research · 2020Article
Corrections and comments
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Authors and funding
15 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brucella spp. are facultative intracellular bacteria that cause zoonotic disease of brucellosis worldwide. Traditional methods for detection of Brucella spp. take 48-72 h that does not meet the need of rapid detection. Herein, a new rapid detection method of Brucella was developed based on polyclonal antibody-conjugating quantum dots and antibody-modified magnetic beads. First, polyclonal antibodies IgG and IgY were prepared and then the antibody conjugated with quantum dots (QDs) and immunomagnetic beads (IMB), respectively, which were activated by N-(3-dimethylaminopropyl)-N'-ethylcar-bodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) to form probes. We used the IMB probe to separate the Brucella and labeled by the QD probe, and then detected the fluorescence intensity with a fluorescence spectrometer. The detection method takes 105 min with a limit of detection of 10
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