Evidence map›Paper›PMID 28282254›Full record

ArticleNucleosides, nucleotides & nucleic acids2017

Synthesis of two fluorescent GTPγS molecules and their biological relevance.

Denise J Trans, Ruoli Bai, J Bennet Addison, Ruiwu Liu, Ernest Hamel, Matthew A Coleman, Paul T Henderson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleosides, nucleotides & nucleic acids, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Denise J Transa Department of Internal Medicine and UC Davis Comprehensive Cancer Center , University of California , Davis , CA , USA.
Ruoli Baib Screening Technologies Branch, Developmental Therapeutics Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute, Frederick National Laboratory for Cancer Research , National Institutes of Health , Frederick , MD , USA.
J Bennet Addisonc Nuclear Magnetic Resonance Facility , University of California , Davis , CA , USA.
Ruiwu Liud Department of Biochemistry and Molecular Medicine , University of California , Davis , CA , USA.
Ernest Hamelb Screening Technologies Branch, Developmental Therapeutics Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute, Frederick National Laboratory for Cancer Research , National Institutes of Health , Frederick , MD , USA.
Matthew A Colemane Department of Radiation Oncology , University of California , Davis , CA , USA.
Paul T Hendersona Department of Internal Medicine and UC Davis Comprehensive Cancer Center , University of California , Davis , CA , USA.

Funding

Staff InvestigatorsP30CA093373 · NCI · UNIVERSITY OF CALIFORNIA DAVIS · PI Laura Marcu · 2002 to 2026
$84.9M
Characterization of Erbb Receptors in NanoparticlesR01CA155642 · NCI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI COLEMAN, MATTHEW ADRIAN, HENDERSON, PAUL THOMAS · 2011 to 2015
$1.6M
NCI NIH HHS P30 CA093373NCI NIH HHS R01 CA155642
6 · The paper itself

Abstract

Fluorescent GTP analogues are utilized for an assortment of nucleic acid and protein characterization studies. Non-hydrolysable analogues such as GTPγS offer the advantage of keeping proteins in a GTP-bound conformation due to their resistance to hydrolysis into GDP. Two novel fluorescent GTPγS molecules were developed by linking fluorescein and tetramethylrhodamine to the γ-thiophosphate of GTPγS. Chemical and biological analysis of these two compounds revealed their successful synthesis and ability to bind to the nucleotide-binding site of tubulin. These two new fluorescent non-hydrolysable nucleotides offer new possibilities for biophysical and biochemical characterization of GTP-binding proteins.

Indexed as

Chemistry Techniques, SyntheticEnergy TransferFluorescent DyesGTP-Binding ProteinsGuanosine 5'-O-(3-Thiotriphosphate)Protein MultimerizationProtein Structure, QuaternaryTubulinFluorescent DyesGTP-Binding ProteinsGuanosine 5'-O-(3-Thiotriphosphate)Tubulinfluorescent nucleotide analogsFRETNMR and tubulin polymerizationNucleoside and nucleotide analogs

Identifiers

PMID28282254
PMCPMC5522600

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.