ArticleScientific reports2017
Visualising recalcitrance by colocalisation of cellulase, lignin and cellulose in pretreated pine biomass using fluorescence microscopy.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Co-Producing Reducing Sugars, Furfural and Xylo-Oligosaccharides from Reeds via Effective Pretreatment by Ternary Deep Eutectic Solvent Lactic Acid:Cetyltrimethylammonium Bromide:CuCl₂.Applied biochemistry and biotechnology · 2026Article
- Advances in understanding dietary fiber: Classification, structural characterization, modification, and gut microbiome interactions.Comprehensive reviews in food science and food safety · 2025Review
- Protocol to analyse the structural composition by fluorescence microscopy and different conventional and fluorescence staining methods.MethodsX · 2024Article
- Lignin impairs Cel7A degradation of in vitro lignified cellulose by impeding enzyme movement and not by acting as a sink.Biotechnology for biofuels and bioproducts · 2024Article
- Spatial activity mapping of ß-mannanase on soybean seeds.Scientific reports · 2024Article
- Fluorescent Imaging of Extracellular Fungal Enzymes Bound onto Plant Cell Walls.International journal of molecular sciences · 2022Article
- Review
- Recent advances in understanding the effects of lignin structural characteristics on enzymatic hydrolysis.Biotechnology for biofuels · 2021Review
- Physical-chemical properties of cell wall interface significantly correlated to the complex recalcitrance of corn straw.Biotechnology for biofuels · 2021Article
- Generation of highly amenable cellulose-Iβ via selective delignification of rice straw using a reusable cyclic ether-assisted deep eutectic solvent system.Scientific reports · 2021Article
- Plant cell wall hydrolysis process reveals structure-activity relationships.Plant methods · 2020Article
- Autofluorescence in Plants.Molecules (Basel, Switzerland) · 2020Review
- Mobility of pectin methylesterase in pectin/cellulose gels is enhanced by the presence of cellulose and by its catalytic capacity.Scientific reports · 2019Article
- Differences in the Structural Chemical Composition of the Primary Xylem of Cactaceae: A Topochemical Perspective.Frontiers in plant science · 2019Article
- Inactivation ofBiotechnology for biofuels · 2019Article
- Exploring the Treasure of Plant Molecules With Integrated Biorefineries.Frontiers in plant science · 2019Article
- Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans.Nature communications · 2018Article
- Comparison of Micropore Distribution in Cell Walls of Softwood and Hardwood Xylem.Plant physiology · 2018Article
- The properties of fibreboard based on nanolignocelluloses/CaCOScientific reports · 2018Article
- Stereochemical identification of glucans by oligothiophenes enables cellulose anatomical mapping in plant tissues.Scientific reports · 2018Article
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2 authors.
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Abstract
Mapping the location of bound cellulase enzymes provides information on the micro-scale distribution of amenable and recalcitrant sites in pretreated woody biomass for biofuel applications. The interaction of a fluorescently labelled cellulase enzyme cocktail with steam-exploded pine (SEW) was quantified using confocal microscopy. The spatial distribution of Dylight labelled cellulase was quantified relative to lignin (autofluorescence) and cellulose (Congo red staining) by measuring their colocalisation using Pearson correlations. Correlations were greater in cellulose-rich secondary cell walls compared to lignin-rich middle lamella but with significant variations among individual biomass particles. The distribution of cellulose in the pretreated biomass accounted for 30% of the variation in the distribution of enzyme after correcting for the correlation between lignin and cellulose. For the first time, colocalisation analysis was able to quantify the spatial distribution of amenable and recalcitrant sites in relation to the histochemistry of cellulose and lignin. This study will contribute to understanding the role of pretreatment in enzymatic hydrolysis of recalcitrant softwood biomass.
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