ArticleBiotechnology letters2017
Modification of fatty acid selectivity of Candida antarctica lipase A by error-prone PCR.
Article in Biotechnology letters, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed, 10 citations in OpenAlex.
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Increased mRNA Stability and Expression Level ofJournal of microbiology and biotechnology · 2021Article
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1 author at 1 institution in 1 country.
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Abstract
objectiveTo generate Candida antarctica lipase A (CAL-A) mutants with modified fatty acid selectivities and improved lipolytic activities using error-prone PCR (epPCR).
resultsA Candida antarctica lipase A mutant was obtained in three rounds of epPCR. This mutant showed a 14 times higher ability to hydrolyze triacylglycerols containing conjugated linoleic acids, and was 12 and 14 times more selective towards cis-9, trans-11 and trans-10, cis-12 isomers respectively, compared to native lipase. Lipolytic activities towards fatty acid esters were markedly improved, in particular towards butyric, lauric, stearic and palmitic esters.
conclusionDirected molecular evolution is an efficient method to generate lipases with desirable selectivity towards CLA isomers and improved lipolytic activities towards esters of fatty acids.
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