ArticleScience (New York, N.Y.)2017
Engineering the ribosomal DNA in a megabase synthetic chromosome.
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Article in Science (New York, N.Y.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
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98citing papers in PubMed
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Who cites it
98 citing papers in PubMed.
- Ribosome engineering enhances genetic code expansion inSynthetic and systems biotechnology · 2027Article
- Synthetic Chromosomes in Yeast: A Platform for Understanding and Programming Eukaryotic Life.Microbial biotechnology · 2026Review
- Advances in large DNA fragment assembly for microbial cell factory engineering.Quantitative biology (Beijing, China) · 2026Review
- Technologies for engineering repetitive DNA.Quantitative biology (Beijing, China) · 2026Review
- High-throughput synthesis of DNA fragments by molecular self-assembly of overlapping oligonucleotides.Nature biotechnology · 2026Article
- Automated linear DNA assembly of A. thaliana's chloroplast and mitochondrial genome.Nucleic acids research · 2026Article
- Synthetic rewriting technologies in mammalian cells.Nature communications · 2026Review
- Inducible chromosomal rearrangement reveals nonlinear polygenic dosage effects in driving aneuploid yeast traits.Nature communications · 2025Article
- Assembly and delivery of large DNA via chromosome elimination in yeast.Nature protocols · 2025Review
- De novo assembly and delivery of synthetic megabase-scale human DNA into mouse early embryos.Nature methods · 2025Article
- The design and engineering of synthetic genomes.Nature reviews. Genetics · 2025Review
- Recent technology advancements in large-scale DNA assembly.Quantitative biology (Beijing, China) · 2025Article
- Construction and iterative redesign of synXVI a 903 kb synthetic Saccharomyces cerevisiae chromosome.Nature communications · 2025Article
- Towards the first synthetic eukaryotic cell.Biosafety and health · 2024Review
- The de novo design and synthesis of yeast chromosome XIII facilitates investigations on aging.Nature communications · 2024Article
- Amplification editing empowersInnovation (Cambridge (Mass.)) · 2024Review
- Methodological advances enabled by the construction of a synthetic yeast genome.Cell reports methods · 2024Article
- Automated high-throughput DNA synthesis and assembly.Heliyon · 2024Review
- A self-regulated network for dynamically balancing multiple precursors in complex biosynthetic pathways.Metabolic engineering · 2024Article
- Recent advances in genome-scale engineering inEngineering microbiology · 2024Review
38 more citing papers are in PubMed but not listed here.
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Authors and funding
44 authors.
Weimin ZhangKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.ORCID 0000-0002-8836-1438
Guanghou ZhaoKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.ORCID 0000-0002-3192-5423
Zhouqing LuoKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.ORCID 0000-0001-5426-5176
Yicong LinKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Lihui WangKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.ORCID 0000-0002-8479-7859
Yakun GuoKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.ORCID 0000-0002-4847-0648
Ann WangKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Shuangying JiangKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Qingwen JiangKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.ORCID 0000-0003-3444-2854
Jianhui GongBGI-Shenzhen, Shenzhen 518083, China.ORCID 0000-0002-3910-9260
Yun WangBGI-Shenzhen, Shenzhen 518083, China.ORCID 0000-0001-5872-3269
Sha HouKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.ORCID 0000-0002-4378-0479
Jing HuangKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Tianyi LiKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Yiran QinKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Junkai DongKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Qin QinKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Jiaying ZhangKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Xinzhi ZouKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Xi HeKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Li ZhaoKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Yibo XiaoKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Meng XuKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Erchao ChengKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.ORCID 0000-0001-7829-1870
Ning HuangKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.ORCID 0000-0001-6857-5386
Tong ZhouKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Yue ShenBGI-Shenzhen, Shenzhen 518083, China.
Roy WalkerSchool of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK.ORCID 0000-0003-0960-4591
Yisha LuoSchool of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK.ORCID 0000-0002-4228-7527
Zheng KuangInstitute for Systems Genetics and Department of Biochemistry and Molecular Pharmacology, New York University Langone Medical Center, New York, NY 10011, USA.ORCID 0000-0001-5855-8371
Leslie A MitchellInstitute for Systems Genetics and Department of Biochemistry and Molecular Pharmacology, New York University Langone Medical Center, New York, NY 10011, USA.ORCID 0000-0001-7869-9817
Kun YangHigh-Throughput Biological Center and Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.ORCID 0000-0002-1060-8082
Sarah M RichardsonHigh-Throughput Biological Center and Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.ORCID 0000-0002-9820-9421
Yi WuKey Laboratory of Systems Bioengineering (Ministry of Education), SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.ORCID 0000-0002-2539-9228
Bing-Zhi LiKey Laboratory of Systems Bioengineering (Ministry of Education), SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.ORCID 0000-0003-4121-3048
Ying-Jin YuanKey Laboratory of Systems Bioengineering (Ministry of Education), SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.ORCID 0000-0003-0553-0089
Huanming YangBGI-Shenzhen, Shenzhen 518083, China.ORCID 0000-0003-1703-3012
Jiwei LinWuxi Qinglan Biotechnology Inc., Yixing, Jiangsu 214200, China.ORCID 0000-0003-3803-6503
Guo-Qiang ChenKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Qingyu WuKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Joel S BaderHigh-Throughput Biological Center and Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.ORCID 0000-0002-6020-4625
Yizhi CaiSchool of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK.ORCID 0000-0003-1663-2865
Jef D BoekeInstitute for Systems Genetics and Department of Biochemistry and Molecular Pharmacology, New York University Langone Medical Center, New York, NY 10011, USA.ORCID 0000-0001-5322-4946
Junbiao DaiKey Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China. jbdai@tsinghua.edu.cn.ORCID 0000-0002-5299-4700
Funding
Biotechnology and Biological Sciences Research Council BB/M005690/1
Abstract
We designed and synthesized a 976,067-base pair linear chromosome, synXII, based on native chromosome XII in
Indexed as
Genome, FungalCell NucleusChromosomes, Artificial, YeastDNA, RibosomalGenetic EngineeringSaccharomyces cerevisiaeSynthetic BiologyTranscriptomeDNA, Ribosomal
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PMID
28280149What OpenQuestion holds
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.