ArticleInvestigative ophthalmology & visual science2017
In Vivo Bioluminescence Imaging for Longitudinal Monitoring of Inflammation in Animal Models of Uveitis.
Article in Investigative ophthalmology & visual science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
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- Novel NF-κB reporter mouse for the non-invasive monitoring of inflammatory diseases.Scientific reports · 2023Article
- Systemic prime exacerbates the ocular immune response to heat-killed Mycobacterium tuberculosis.Experimental eye research · 2022Article
- Recent advances in optical coherence tomography for anterior segment imaging in small animals and their clinical implications.The ocular surface · 2022Review
- Protocols for the Evaluation of a Lymphatic Drug Delivery System Combined with Bioluminescence to Treat Metastatic Lymph Nodes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis.Journal of visualized experiments : JoVE · 2021Article
- In vivo optical molecular imaging of inflammation and immunity.Journal of molecular medicine (Berlin, Germany) · 2021Review
- Glucan particles as a novel adjuvant for the induction of experimental autoimmune encephalomyelitis.Cellular immunology · 2021Article
- CLEC-1 Acts as a Negative Regulator of Dectin-1 Induced Host Inflammatory Response Signature in Aspergillus fumigatus Keratitis.Investigative ophthalmology & visual science · 2021Article
- Characterization of Gene Therapy Associated Uveitis Following Intravitreal Adeno-Associated Virus Injection in Mice.Investigative ophthalmology & visual science · 2021Article
- Quantitative Assessment of Experimental Ocular Inflammatory Disease.Frontiers in immunology · 2021Review
- Non-invasive multimodal imaging of Diabetic Retinopathy: A survey on treatment methods and Nanotheranostics.Nanotheranostics · 2021Article
- In-vivo imaging revealed antigen-directed gingival B10 infiltration in experimental periodontitis.Biochimica et biophysica acta. Molecular basis of disease · 2021Article
- The Anti-Inflammatory Effect of KS23, A Novel Peptide Derived From Globular Adiponectin, on Endotoxin-Induced Uveitis in Rats.Frontiers in pharmacology · 2020Article
- Novel window for cancer nanotheranostics: non-invasive ocular assessments of tumor growth and nanotherapeutic treatment efficacyBiomedical optics express · 2019Article
- Comparison of Aqueous and Vitreous Lymphocyte Populations From Two Rat Models of Experimental Uveitis.Investigative ophthalmology & visual science · 2018Article
- Bioluminescence Imaging of Matrix Metalloproteinases-2 and -9 Activities in Ethanol-injured Cornea of Mice.In vivo (Athens, Greece)Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Purpose: We develop a quantitative bioluminescence assay for in vivo longitudinal monitoring of inflammation in animal models of uveitis. Methods: Three models of experimental uveitis were induced in C57BL/6 albino mice: primed mycobacterial uveitis (PMU), endotoxin-induced uveitis (EIU), and experimental autoimmune uveitis (EAU). Intraperitoneal injection of luminol sodium salt, which emits light when oxidized, provided the bioluminescence substrate. Bioluminescence images were captured by a PerkinElmer In Vivo Imaging System (IVIS) Spectrum and total bioluminescence was analyzed using Living Image software. Bioluminescence on day zero was compared to bioluminescence on the day of peak inflammation for each model. Longitudinal bioluminescence imaging was performed in EIU and EAU. Results: In the presence of luminol, intraocular inflammation generates detectable bioluminescence in three mouse models of uveitis. Peak bioluminescence in inflamed PMU eyes (1.46 × 105 photons/second [p/s]) was significantly increased over baseline (1.47 × 104 p/s, P = 0.01). Peak bioluminescence in inflamed EIU eyes (3.18 × 104 p/s) also was significantly increased over baseline (1.09 × 104 p/s, P = 0.04), and returned to near baseline levels by 48 hours. In EAU, there was a nonsignificant increase in bioluminescence at peak inflammation. Conclusions: In vivo bioluminescence may be used as a noninvasive, quantitative measure of intraocular inflammation in animal models of uveitis. Primed mycobacterial uveitis and EIU are both acute models with robust anterior inflammation and demonstrated significant changes in bioluminescence corresponding with peak inflammation. Experimental autoimmune uveitis is a more indolent posterior uveitis and generated a more modest bioluminescent signal. In vivo imaging system bioluminescence is a nonlethal, quantifiable assay that can be used for monitoring inflammation in animal models of uveitis.
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