ReviewRNA biology2017
Antibodies specific for nucleic acid modifications.
Review in RNA biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Investigating RNA dynamics from single molecule transcriptomes.Trends in genetics : TIG · 2025Review
- Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide.Nucleic acids research · 2025Article
- Comparative analysis of improved m6A sequencing based on antibody optimization for low-input samples.Scientific reports · 2025Article
- Spatial mapping of the DNA adducts in cancer.DNA repair · 2023Review
- Harnessing Nature's Molecular Recognition Capabilities to Map and Study RNA Modifications.Accounts of chemical research · 2022Article
- Nanopore sequencing and de novo assembly of a misidentified Camelpox vaccine reveals putative epigenetic modifications and alternate protein signal peptides.Scientific reports · 2021Article
- Analysis of RNA Modifications by Second- and Third-Generation Deep Sequencing: 2020 Update.Genes · 2021Review
- May the Odds Be Ever in Your Favor: Non-deterministic Mechanisms Diversifying Cell Surface Molecule Expression.Frontiers in cell and developmental biology · 2021Review
- Validation strategies for antibodies targeting modified ribonucleotides.RNA (New York, N.Y.) · 2020Article
- Post-transcriptional air pollution oxidation to the cholesterol biosynthesis pathway promotes pulmonary stress phenotypes.Communications biology · 2020Article
- No evidence for DNAScience advances · 2020Article
- Opportunities and challenges in long-read sequencing data analysis.Genome biology · 2020Review
- Discovery of a new predominant cytosine DNA modification that is linked to gene expression in malaria parasites.Nucleic acids research · 2020Article
- Mapping and editing of nucleic acid modifications.Computational and structural biotechnology journal · 2020Review
- Limited antibody specificity compromises epitranscriptomic analyses.Nature communications · 2019Article
- Development and validation of monoclonal antibodies against N6-methyladenosine for the detection of RNA modifications.PloS one · 2019Article
- Detection and analysis of RNA methylation.F1000Research · 2019Review
- Emerging approaches for detection of methylation sites in RNA.Open biology · 2018Review
- Editorial: RNA modifications - what to read first?RNA biology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleotide modifications constitute marks in RNA and DNA that contribute to gene regulation, development and other cellular processes. The understanding of their intricate molecular roles has been hampered by the high number of different modifications, the lack of effective methods and tools for their detection and quantification as well as by their complex structure-function relationship. The recent development of RNA and DNA immunoprecipitation followed by high-throughput sequencing (RIP- and DIP-seq) initiated detailed transcriptome- and genome-wide studies. Both techniques depend on highly specific and sensitive antibodies to specifically enrich the targeted modified nucleotides without background or potential biases. Here, we review the challenges and developments when generating and validating antibodies targeting modified nucleotides. We discuss antibody-antigen interactions, different strategies of antigen generation and compare different binder formats suitable for state-of-the-art high resolution mapping and imaging technologies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.