ArticleRNA biology2017
Both exo- and endo-nucleolytic activities of RNase J1 from Staphylococcus aureus are manganese dependent and active on triphosphorylated 5'-ends.
Article in RNA biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Metastable Folding of Bacillus subtilis glmS Ribozyme Modulates Turnover by RNase J1.Journal of molecular biology · 2026Article
- Acetylation regulates the oligomerization state and activity of RNase J, the Helicobacter pylori major ribonuclease.Nature communications · 2023Article
- Factors impacting the regulation ofMicrobiology spectrum · 2023Article
- PerR-Regulated Manganese Import Contributes to Oxidative Stress Defense in Streptococcus suis.Applied and environmental microbiology · 2022Article
- RNase J1 and J2 Are Host-Encoded Factors for Plasmid Replication.Frontiers in microbiology · 2021Article
- Diversity and Versatility in Small RNA-Mediated Regulation in Bacterial Pathogens.Frontiers in microbiology · 2021Review
- Structural and biochemical characteristics of twoThe Journal of biological chemistry · 2020Article
- Regulation of mRNA Stability During Bacterial Stress Responses.Frontiers in microbiology · 2020Review
- Article
- Manganese Detoxification by MntE Is Critical for Resistance to Oxidative Stress and Virulence ofmBio · 2019Article
- The Arabidopsis chloroplast RNase J displays both exo- and robust endonucleolytic activities.Plant molecular biology · 2019Article
- Tracking the elusive 5' exonuclease activity of Chlamydomonas reinhardtii RNase J.Plant molecular biology · 2018Article
- RNases and Helicases in Gram-Positive Bacteria.Microbiology spectrum · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA decay and RNA maturation are important steps in the regulation of bacterial gene expression. RNase J, which is present in about half of bacterial species, has been shown to possess both endo- and 5' to 3' exo-ribonuclease activities. The exonucleolytic activity is clearly involved in the degradation of mRNA and in the maturation of at least the 5' end of 16S rRNA in the 2 Firmicutes Staphylococcus aureus and Bacillus subtilis. The endoribonuclease activity of RNase J from several species has been shown to be weak in vitro and 3-D structural data of different RNase J orthologs have not provided a clear explanation for the molecular basis of this activity. Here, we show that S. aureus RNase J1 is a manganese dependent homodimeric enzyme with strong 5' to 3' exo-ribonuclease as well as endo-ribonuclease activity. In addition, we demonstrated that SauJ1 can efficiently degrade 5' triphosphorylated RNA. Our results highlight RNase J1 as an important player in RNA turnover in S. aureus.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.