ArticleThe New phytologist2018
Real-time detection of N-end rule-mediated ubiquitination via fluorescently labeled substrate probes.
Article in The New phytologist, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Structural basis for the recognition and ubiquitylation of type-2 N-degron substrate by PRT1 plant N-recognin.Nature communications · 2025Article
- Ubiquitin E3 ligases in the plant Arg/N-degron pathway.The Biochemical journal · 2024Review
- The SMXL8-AGL9 module mediates crosstalk between strigolactone and gibberellin to regulate strigolactone-induced anthocyanin biosynthesis in apple.The Plant cell · 2024Article
- BIG enhances Arg/N-degron pathway-mediated protein degradation to regulate Arabidopsis hypoxia responses and suberin deposition.The Plant cell · 2024Article
- The lowdown on breakdown: Open questions in plant proteolysis.The Plant cell · 2024Review
- Ubiquitination detection techniques.Experimental biology and medicine (Maywood, N.J.) · 2023Review
- Using CRL3Plant direct · 2022Article
- Trichome Transcripts as Efficiency Control for Synthetic Biology and Molecular Farming.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A Yeast-Based Functional Assay to Study Plant N-Degron - N-Recognin Interactions.Frontiers in plant science · 2021Article
- Assays and technologies for developing proteolysis targeting chimera degraders.Future medicinal chemistry · 2020Review
- ThePlant direct · 2019Article
- Tandem Fluorescent Protein Timers for Noninvasive Relative Protein Lifetime Measurement in Plants.Plant physiology · 2019Article
- New beginnings and new ends: methods for large-scale characterization of protein termini and their use in plant biology.Journal of experimental botany · 2019Review
- Modulating Protein Stability to Switch Toxic Protein Function On and Off in Living Cells.Plant physiology · 2019Article
- Visualizing ubiquitination in mammalian cells.EMBO reports · 2019Review
- A High-Throughput Assay for Monitoring Ubiquitination in Real Time.Frontiers in chemistry · 2019Article
- Genetic interactions between ABA signalling and the Arg/N-end rule pathway during Arabidopsis seedling establishment.Scientific reports · 2018Article
- Redox control and autoxidation of class 1, 2 and 3 phytoglobins from Arabidopsis thaliana.Scientific reports · 2018Article
- Insights into degradation mechanism of N-end rule substrates by p62/SQSTM1 autophagy adapter.Nature communications · 2018Article
- N-terminomics reveals control of Arabidopsis seed storage proteins and proteases by the Arg/N-end rule pathway.The New phytologist · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The N-end rule pathway has emerged as a major system for regulating protein functions by controlling their turnover in medical, animal and plant sciences as well as agriculture. Although novel functions and enzymes of the pathway have been discovered, the ubiquitination mechanism and substrate specificity of N-end rule pathway E3 ubiquitin ligases have remained elusive. Taking the first discovered bona fide plant N-end rule E3 ligase PROTEOLYSIS1 (PRT1) as a model, we used a novel tool to molecularly characterize polyubiquitination live, in real time. We gained mechanistic insights into PRT1 substrate preference and activation by monitoring live ubiquitination using a fluorescent chemical probe coupled to artificial substrate reporters. Ubiquitination was measured by rapid in-gel fluorescence scanning as well as in real time by fluorescence polarization. The enzymatic activity, substrate specificity, mechanisms and reaction optimization of PRT1-mediated ubiquitination were investigated ad hoc instantaneously and with significantly reduced reagent consumption. We demonstrated that PRT1 is indeed an E3 ligase, which has been hypothesized for over two decades. These results demonstrate that PRT1 has the potential to be involved in polyubiquitination of various substrates and therefore pave the way to understanding recently discovered phenotypes of prt1 mutants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.