ArticleRedox report : communications in free radical research2017
Vitamin C and Trolox decrease oxidative stress and hemolysis in cold-stored human red blood cells.
Article in Redox report : communications in free radical research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Biotransformation of medicarpin from homopterocarpin by Aspergillus niger and its biological characterization.Scientific reports · 2025Article
- The vitamin C paradigm: new frontiers in blood transfusion.Annals of medicine and surgery (2012) · 2025Review
- Synergistic Effects of a Novel Combination of Natural Compounds Prevent HInternational journal of molecular sciences · 2025Article
- Biopreservation and reversal of oxidative injury during blood storage by a novel curcumin-based gel formulation.Scientific reports · 2024Article
- Extended supercooled storage of red blood cells.Communications biology · 2024Article
- Biopreservation and Reversal of Oxidative Injury During Blood Storage by a Novel Curcumin-based Gel Formulation.Research square · 2024Article
- Mechanisms of ferroptosis and the relationship between ferroptosis and ER stress after JEV and HSV infection.Frontiers in microbiology · 2024Review
- Molecular modifications to mitigate oxidative stress and improve red blood cell storability.Frontiers in physiology · 2024Review
- Ascorbic acid enhances the cold preservation period of human adipose tissue-derived mesenchymal stromal cells.Regenerative therapy · 2023Article
- Silk Fibroin Particles as Carriers in the Development of Hemoglobin-Based Oxygen Carriers.Advanced nanobiomed research · 2023Article
- Inhibition of Ferroptosis Enables Safe Rewarming of HEK293 Cells following Cooling in University of Wisconsin Cold Storage Solution.International journal of molecular sciences · 2023Article
- Silk Fibroin Particles as Carriers in the Development of All-Natural Hemoglobin-Based Oxygen Carriers (HBOCs).bioRxiv : the preprint server for biology · 2023Article
- Changes in hemoglobin oxidation and band 3 during blood storage impact oxygen sensing and mitochondrial bioenergetic pathways in the human pulmonary arterial endothelial cell model.Frontiers in physiology · 2023Article
- Article
- Protective Effect of Dinitrosyl Iron Complexes with Glutathione in Red Blood Cell Lysis Induced by Hypochlorous Acid.Oxidative medicine and cellular longevity · 2019Article
- Metabolic effect of alkaline additives and guanosine/gluconate in storage solutions for red blood cells.Transfusion · 2018Article
- Attenuation of Red Blood Cell Storage Lesions with Vitamin C.Antioxidants (Basel, Switzerland) · 2017Article
- Antioxidative effects of α-tocopherol on stored human red blood cell units.Asian journal of transfusion scienceArticle
- Effect of N-Acetylcysteine, Ascorbic Acid, and a Vitamin E Analog on Oxidative and Storage Lesions in Canine Packed Red Blood Cells.Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)Article
- Salvianolic acid B alleviates red blood cell storage lesion with GPX4 preservation and reduced lipid peroxidation.Blood transfusion = Trasfusione del sangueArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesTo investigate the effects of sodium ascorbate (SA) (5-3125 μM) and a combination of SA and Trolox (25 and 125 μM) on oxidative changes generated in red blood cells (RBCs) followed by up to 20 days refrigerated storage.
methodsRBCs were isolated from CPD-preserved human blood. Percentage of hemolysis and extracellular activity of lactate dehydrogenase (LDH) were measured to assess the RBC membrane integrity. Lipid peroxidation (LPO), glutathione (GSH) and total antioxidant capacity (TAC) were quantified by thiobarbituric acid-reactive substances, Ellman's reagent and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) [Formula: see text]-based assay, respectively.
resultsSA failed to reduce the storage-induced hemolysis and RBC membrane permeability. Addition of SA resulted in a concentration-independent LPO inhibition and increased TAC. A combination of SA/Trolox supplemented to the RBC medium significantly inhibited hemolysis, LDH leakage, LPO, GSH depletion and enhanced TAC. DISCUSSION: The effects of vitamin C action are closely concentration-dependent and may be modulated by a variety of compounds (e.g. Hb degradation products) released from RBCs during the prolonged storage, changing its properties from anti- to pro-oxidative. The two different class antioxidants (SA/Trolox) could possibly cooperate to be good potential RBC storage additives ensuring both antiradical and membrane stabilizing protection.
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