Evidence map›Paper›PMID 28274807›Full record

ArticleThe Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases

Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in Brazil.

Rosana Macedo de Almeida, André Victor Barbosa, Rodrigo de Castro Lisbôa, André Felipe das Mercês Santos, Ernesto Hofer, Deyse Christina Vallim, Cristina Barroso Hofer

Open access · goldAbstract read
In one paragraph

Article in The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
2.1field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 21 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Genomic Characterization of ClinicalFrontiers in microbiology · 2021
    Article
  6. Article
  7. Isolation and Characterization of ClinicalFrontiers in microbiology · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Rosana Macedo de AlmeidaInstituto Oswaldo Cruz (Fiocruz), Laboratório de Zoonoses Bacterianas, Rio de Janeiro, RJ, Brazil; Universidade Federal do Rio de Janeiro (UFRJ), Faculdade de Medicina, Programa de Pós-Graduação em Doenças Infecciosas e Parasitárias, Rio de Janeiro, RJ, Brazil. Electronic address: rosanaalmeida1301@hotmail.com.
André Victor BarbosaInstituto Oswaldo Cruz (Fiocruz), Laboratório de Zoonoses Bacterianas, Rio de Janeiro, RJ, Brazil; Universidade Federal Fluminense, Instituto Biomédico, Laboratório de Enteropatógenos Bacterianos, Microbiologia Veterinária e de Alimentos, Niterói, RJ, Brazil.
Rodrigo de Castro LisbôaInstituto Oswaldo Cruz (Fiocruz), Laboratório de Zoonoses Bacterianas, Rio de Janeiro, RJ, Brazil.
André Felipe das Mercês SantosInstituto Oswaldo Cruz (Fiocruz), Laboratório de Enterobactérias, Rio de Janeiro, RJ, Brazil.
Ernesto HoferInstituto Oswaldo Cruz (Fiocruz), Laboratório de Zoonoses Bacterianas, Rio de Janeiro, RJ, Brazil.
Deyse Christina VallimInstituto Oswaldo Cruz (Fiocruz), Laboratório de Zoonoses Bacterianas, Rio de Janeiro, RJ, Brazil.
Cristina Barroso HoferUniversidade Federal do Rio de Janeiro (UFRJ), Faculdade de Medicina, Programa de Pós-Graduação em Doenças Infecciosas e Parasitárias, Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz · BRUniversidade Federal do Rio de Janeiro · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The herein presented assay provided a bacteriological and molecular characterization of 100 samples of L. monocytogenes isolated from human (43) and food (57) sources, from several regions of Brazil, and collected between 1975 and 2013. Antigenic characterization defined 49% of serotype 4b samples, followed by 28% of serotype 1/2b, 14% of serotype 1/2c, 8% of serotype 1/2a, and 1% of serotype 3b. Both type of samples from human and food origin express the same serotype distribution. Multiplex PCR analysis showed 13 strains of type 4b with the amplification profile 4b-VI (Variant I). Virulence genes hly, inlA, inlB, inlC, inlJ, actA, plcA, and prfA were detected in all samples, highlighting a deletion of 105pb on the actA gene in 23% of serotype 4b samples. Macrorestriction profile with ApaI at PFGE showed 55 pulsotypes, with the occurrence of the same pulsotype in hospitalized patients in São Paulo in 1992 and 1997, and two other highly related pulsotypes in patients hospitalized in Rio de Janeiro in 2008. Recognized pulsotypes in listeriosis cases have also been detected in food. Thus, the prevalence of a serotype and the persistence of certain pulsotypes herald future problems.

Indexed as

Food MicrobiologyBrazilElectrophoresis, Gel, Pulsed-FieldGenes, BacterialHumansListeria monocytogenesMolecular TypingMultiplex Polymerase Chain ReactionSerotypingVirulence FactorsVirulence FactorsBrazilFoodListeria monocytogenesListeriosisPFGEVirulence

Identifiers

PMID28274807
PMCPMC9427587
OpenAlexW2594774656

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.