Evidence map›Paper›PMID 28274217›Full record

ArticleBMC microbiology2017

Disruption of rcsB by a duplicated sequence in a curli-producing Escherichia coli O157:H7 results in differential gene expression in relation to biofilm formation, stress responses and metabolism.

V K Sharma, D O Bayles, D P Alt, T Looft, B W Brunelle, J A Stasko

Open access · goldAbstract read
In one paragraph

Article in BMC microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 24 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

V K SharmaFood Safety and Enteric Pathogens Research Unit, National Animal Disease Center, ARS-USDA, P. O. Box 70, 1920 Dayton Avenue, Ames, IA, 50010, USA. vijay.sharma@ars.usda.gov.
D O BaylesInfectious Bacterial Diseases Research Unit, National Animal Disease Center, ARS-USDA, Ames, IA, 50010, USA.
D P AltInfectious Bacterial Diseases Research Unit, National Animal Disease Center, ARS-USDA, Ames, IA, 50010, USA.
T LooftFood Safety and Enteric Pathogens Research Unit, National Animal Disease Center, ARS-USDA, P. O. Box 70, 1920 Dayton Avenue, Ames, IA, 50010, USA.
B W BrunelleFood Safety and Enteric Pathogens Research Unit, National Animal Disease Center, ARS-USDA, P. O. Box 70, 1920 Dayton Avenue, Ames, IA, 50010, USA.
J A StaskoMicroscopy Services Unit, National Animal Disease Center, ARS-USDA, Ames, IA, 50010, USA.
National Animal Disease Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEscherichia coli O157:H7 (O157) strain 86-24, linked to a 1986 disease outbreak, displays curli- and biofilm-negative phenotypes that are correlated with the lack of Congo red (CR) binding and formation of white colonies (CR

resultsTo identify genes controlling differential expression of curli fimbriae and biofilm formation, the RNA-Seq profile of a CR

conclusionsThe results of this study indicate that RcsB is a global regulator affecting bacterial survival in growth-restrictive environments through upregulation of genes promoting biofilm formation while downregulating certain metabolic functions. Understanding whether rcsB inactivation enhances persistence and survival of O157 in carrier animals and the environment would be important in developing strategies for controlling this bacterial pathogen in these niches.

Indexed as

Amino AcidsAmino Acid SequenceBacterial AdhesionBase SequenceBiofilmsCongo RedCulture MediaDNA, BacterialDNA, RecombinantDown-RegulationEscherichia coli O157Escherichia coli ProteinsFimbriae, BacterialGene Expression Regulation, BacterialGenes, BacterialGenetic Complementation TestAmino Acidscolanic acidCongo RedCpxP protein, E coliCulture MediaDNA, BacterialDNA, RecombinantEscherichia coli ProteinsHeat-Shock ProteinsMembrane ProteinsPolysaccharidesRcsB protein, E coliRNA, BacterialTranscription FactorsBiofilmsCurliEHECStress signaling

Identifiers

PMID28274217
PMCPMC5343319
OpenAlexW2591950106

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.