ArticleBMC microbiology2017
Rapid detection of carbapenemase-producing Klebsiella pneumoniae strains derived from blood cultures by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS).
Article in BMC microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- The Microbiological Characteristics and Genomic Surveillance of Carbapenem-ResistantMicroorganisms · 2025Article
- Bacterial discrimination by Fourier transform infrared spectroscopy, MALDI-mass spectrometry and whole-genome sequencing.Future microbiology · 2024Article
- A rapid MALDI-TOF mass spectrometry-based method for colistin susceptibility testing in Escherichia coli.Microbial biotechnology · 2022Article
- Detection of carbapenemase-producing Enterobacterales by means of matrix-assisted laser desorption ionization time-of-flight mass spectrometry with ertapenem susceptibility-testing disks as source of carbapenem substrate.Frontiers in microbiology · 2022Article
- A rapid method for detecting and distinguishing metallo-β-lactamase-and serine carbapenemase-producingFrontiers in microbiology · 2022Article
- Rapid Detection of Carbapenem-ResistantInfection and drug resistance · 2022Article
- Carbapenemase ProducingAntibiotics (Basel, Switzerland) · 2021Article
- MALDI-TOF MS: An alternative approach for ribotyping Clostridioides difficile isolates in Brazil.Anaerobe · 2021Article
- A Rapid Antimicrobial Susceptibility Test forInfection and drug resistance · 2021Article
- Combination of mass spectrometry and DNA sequencing for detection of antibiotic resistance in diagnostic laboratories.Folia microbiologica · 2020Review
- Detection of carbapenemase producers by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS).European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Review
- Identification of the OXA-48 Carbapenemase Family by Use of Tryptic Peptides and Liquid Chromatography-Tandem Mass Spectrometry.Journal of clinical microbiology · 2019Article
- Evaluation of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identifying VIM- and SPM-type metallo-β-lactamase-producingInfection and drug resistance · 2019Article
- Typing and Species Identification of Clinical Klebsiella Isolates by Fourier Transform Infrared Spectroscopy and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.Journal of clinical microbiology · 2018Article
- MALDI-TOF mass spectrometry technology for detecting biomarkers of antimicrobial resistance: current achievements and future perspectives.Annals of translational medicine · 2018Review
- A Full MALDI-Based Approach to Detect Plasmid-Encoded KPC-ProducingFrontiers in microbiology · 2018Article
- Carbapenem Resistance: A Review.Medical sciences (Basel, Switzerland) · 2017Review
- Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Use with Positive Blood Cultures: Methodology, Performance, and Optimization.Journal of clinical microbiology · 2017Review
- Semi-quantitative MALDI-TOF for antimicrobial susceptibility testing in Staphylococcus aureus.PloS one · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCarbapenemase-producing Enterobacteriaceae (CPE), particularly carbapenemase-producing Klebsiella pneumoniae isolates, are important causative agents of nosocomial infections associated with significant mortality rates mostly in critical wards. The rapid detection and typing of these strains is critical either for surveillance purposes and to prevent outbreaks and optimize antibiotic therapy. In this study, the MALDI-TOF MS method was used to detect rapidly these isolates from blood cultures (BCs) and to obtain proteomic profiles enable to discriminate between carbapenemase-producing and non-carbapenemase-producing strains.
resultsFifty-five K. pneumoniae strains were tested. Identification and carbapenemase-production detection assay using Ertapenem were performed both from bacterial pellets extracted directly from BCs flasks and from subcultures of these strains. For all isolates, a complete antimicrobial susceptibility testing and a genotypic characterization were performed. We found 100% agreement between the carbapenemase-producing profile generated by MALDI TOF MS and that obtained using conventional methods. The assay detected and discriminated different carbapenemase-producing K. pneumoniae isolates within 30 min to 3 h after incubation with Ertapenem.
conclusionsMALDI-TOF MS is a promising, rapid and economical method for the detection of carbapenemase-producing K. pneumoniae strains that could be successfully introduced into the routine diagnostic workflow of clinical microbiology laboratories.
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