ArticlePloS one2017
Functional metagenomics reveals novel β-galactosidases not predictable from gene sequences.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Decoding dietary pectin: from structural complexity and microbial CAZyme-PUL networks to cross-feeding and host-beneficial metabolites.Gut microbes · 2026Review
- Structure and function of a β-1,2-galactosidase from Bacteroides xylanisolvens, an intestinal bacterium.Communications biology · 2025Article
- High-throughput nanopore DNA sequencing of large insert fosmid clones directly from bacterial colonies.Applied and environmental microbiology · 2024Article
- Genetic and functional diversity of β-N-acetylgalactosamine-targeting glycosidases expanded by deep-sea metagenome analysis.Nature communications · 2024Article
- Isolation of Genes Encoding Carbon Metabolism Pathways from Complex Microbial Communities.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Function and Structure ofJournal of applied glycoscience · 2023Article
- Gastrointestinal tract and neuroendocrine system responses of young turkeys to the early administration of antibiotics or feeding a diet containing a coccidiostat.Poultry science · 2022Article
- Functional metagenomic screening identifies an unexpected β-glucuronidase.Nature chemical biology · 2022Article
- Cloning, Expression, Purification, and Characterization of β-Galactosidase fromMolecules (Basel, Switzerland) · 2022Article
- A novel salt-tolerant GH42 β-galactosidase with transglycosylation activity from deep-sea metagenome.World journal of microbiology & biotechnology · 2022Article
- Three Diverse Granule Preparation Methods for Proteomic Analysis of Mature Rice (Molecules (Basel, Switzerland) · 2022Article
- Bioprospecting of microbial enzymes: current trends in industry and healthcare.Applied microbiology and biotechnology · 2022Review
- Metagenomic identification, purification and characterisation of the Bifidobacterium adolescentis BgaC β-galactosidase.Applied microbiology and biotechnology · 2021Article
- Advances in Metagenomics and Its Application in Environmental Microorganisms.Frontiers in microbiology · 2021Review
- β-Galactosidases from a Sequence-Based Metagenome: Cloning, Expression, Purification and Characterization.Microorganisms · 2020Article
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- Xylanolytic Extremozymes Retrieved From Environmental Metagenomes: Characteristics, Genetic Engineering, and Applications.Frontiers in microbiology · 2020Review
- Discovery of Novel Biosynthetic Gene Cluster Diversity From a Soil Metagenomic Library.Frontiers in microbiology · 2020Article
- Novel 'Bacteriospray' Method Facilitates the Functional Screening of Metagenomic Libraries for Antimicrobial Activity.Methods and protocols · 2019Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The techniques of metagenomics have allowed researchers to access the genomic potential of uncultivated microbes, but there remain significant barriers to determination of gene function based on DNA sequence alone. Functional metagenomics, in which DNA is cloned and expressed in surrogate hosts, can overcome these barriers, and make important contributions to the discovery of novel enzymes. In this study, a soil metagenomic library carried in an IncP cosmid was used for functional complementation for β-galactosidase activity in both Sinorhizobium meliloti (α-Proteobacteria) and Escherichia coli (γ-Proteobacteria) backgrounds. One β-galactosidase, encoded by six overlapping clones that were selected in both hosts, was identified as a member of glycoside hydrolase family 2. We could not identify ORFs obviously encoding possible β-galactosidases in 19 other sequenced clones that were only able to complement S. meliloti. Based on low sequence identity to other known glycoside hydrolases, yet not β-galactosidases, three of these ORFs were examined further. Biochemical analysis confirmed that all three encoded β-galactosidase activity. Lac36W_ORF11 and Lac161_ORF7 had conserved domains, but lacked similarities to known glycoside hydrolases. Lac161_ORF10 had neither conserved domains nor similarity to known glycoside hydrolases. Bioinformatic and structural modeling implied that Lac161_ORF10 protein represented a novel enzyme family with a five-bladed propeller glycoside hydrolase domain. By discovering founding members of three novel β-galactosidase families, we have reinforced the value of functional metagenomics for isolating novel genes that could not have been predicted from DNA sequence analysis alone.
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