ArticleScientific reports2017
Scrutiny of Mycobacterium tuberculosis 19 kDa antigen proteoforms provides new insights in the lipoglycoprotein biogenesis paradigm.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Metabolic Tagging Reveals Surface-Associated Lipoproteins in Mycobacteria.ACS infectious diseases · 2025Article
- Metabolic tagging reveals surface-associated lipoproteins in mycobacteria.bioRxiv : the preprint server for biology · 2025Article
- Fitness costs of Mycobacterium tuberculosis resistant to rifampicin is compensated by rapid Th2 polarization mediated by early and high IL-4 production during mice infection.Scientific reports · 2025Article
- An anti-LpqH human monoclonal antibody from an asymptomatic individual mediates protection against Mycobacterium tuberculosis.NPJ vaccines · 2023Article
- Article
- Bacillus Calmette-Guérin-Induced Human Mast Cell Activation Relies on IL-33 Priming.International journal of molecular sciences · 2022Article
- Immunochemistry-Based Diagnosis of Extrapulmonary Tuberculosis: A Strategy for Large-Scale Production of MPT64-Antibodies for Use in the MPT64 Antigen Detection Test.Antibodies (Basel, Switzerland) · 2021Article
- Methods for Proteomic Analyses of Mycobacteria.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Targeted Lipidomics of Mycobacterial Lipids and Glycolipids.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Evolution of Drug-ResistantFrontiers in microbiology · 2021Review
- Structural basis of trehalose recycling by the ABC transporter LpqY-SugABC.Science advances · 2020Article
- Potential Plasticity of the Mannoprotein Repertoire Associated toMolecules (Basel, Switzerland) · 2020Article
- Article
- Integrative proteomic and glycoproteomic profiling of Mycobacterium tuberculosis culture filtrate.PloS one · 2020Article
- Mycobacterium abscessus virulence traits unraveled by transcriptomic profiling in amoeba and macrophages.PLoS pathogens · 2019Article
- Proteomic Analysis of Antigen 60 Complex ofVaccines · 2019Article
- Ample glycosylation in membrane and cell envelope proteins may explain the phenotypic diversity and virulence in the Mycobacterium tuberculosis complex.Scientific reports · 2019Article
- VisioProt-MS: interactive 2D maps from intact protein mass spectrometry.Bioinformatics (Oxford, England) · 2019Article
- Top-Down and Intact Protein Mass Spectrometry Data Visualization for Proteoform Analysis Using VisioProt-MS.Bioinformatics and biology insights · 2019Article
- Top-Down Proteomics: Ready for Prime Time?Analytical chemistry · 2018Review
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Post-translational modifications (PTMs) are essential processes conditioning the biophysical properties and biological activities of the vast majority of mature proteins. However, occurrence of several distinct PTMs on a same protein dramatically increases its molecular diversity. The comprehensive understanding of the functionalities resulting from any particular PTM association requires a highly challenging full structural description of the PTM combinations. Here, we report the in-depth exploration of the natural structural diversity of the M. tuberculosis (Mtb) virulence associated 19 kDa lipoglycoprotein antigen (LpqH) using intact protein high-resolution mass spectrometry (HR-MS) coupled to liquid chromatography. Combined top-down and bottom-up HR-MS analyses of the purified Mtb LpqH protein allow, for the first time, to uncover a complex repertoire of about 130 molecular species resulting from the intrinsically heterogeneous combination of lipidation and glycosylation together with some truncations. Direct view on the co-occurring PTMs stoichiometry reveals the presence of functionally distinct LpqH lipidation states and indicates that glycosylation is independent from lipidation. This work allowed the identification of a novel unsuspected phosphorylated form of the unprocessed preprolipoglycoprotein totally absent from the current lipoglycoprotein biogenesis pathway and providing new insights into the biogenesis and functional determinants of the mycobacterial lipoglycoprotein interacting with the host immune PRRs.
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