ArticleLasers in medical science2017
Quantifying glucose and lipid components in human serum by Raman spectroscopy and multivariate statistics.
Article in Lasers in medical science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- Comparison of different Raman spectrometer models in the quantification of blood serum analytes.Lasers in medical science · 2025Article
- Plasma infrared fingerprinting with machine learning enables single-measurement multi-phenotype health screening.Cell reports. Medicine · 2024Article
- Differences in whole blood before and after hemodialysis session of subjects with chronic kidney disease measured by Raman spectroscopy.Lasers in medical science · 2024Article
- A Raman spectral marker for the iso-octyl chain structure of cholesterol.Analytical science advances · 2024Article
- Raman Spectroscopy and Cystic Fibrosis Disease: An Alternative Potential Tool for Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Modulator Response Differentiation-A Pilot Study Based on Serum Samples.Molecules (Basel, Switzerland) · 2024Article
- Central and Peripheral Fatigue Evaluation during Physical Exercise in Athletic Horses by Means of Raman Spectroscopy.Animals : an open access journal from MDPI · 2023Article
- Label-free atherosclerosis diagnosis through a blood drop of apolipoprotein E knockout mouse model using surface-enhanced Raman spectroscopy validated by machine learning algorithm.Bioengineering & translational medicine · 2023Article
- Raman spectroscopy and sciatic functional index (SFI) after low-level laser therapy (LLLT) in a rat sciatic nerve crush injury model.Lasers in medical science · 2022Article
- Diagnosing COVID-19 in human serum using Raman spectroscopy.Lasers in medical science · 2022Article
- Analytical performance of Raman spectroscopy in assaying biochemical components in human serum.Lasers in medical science · 2022Article
- The interaction of chondroitin sulfate with a lipid monolayer observed by using nonlinear vibrational spectroscopy.Physical chemistry chemical physics : PCCP · 2021Article
- Comparison of Surface-Enhanced Raman Scattering Properties of Serum and Urine for the Detection of Chronic Kidney Disease in Patients.Applied spectroscopy · 2021Article
- Diagnosing sickle cell disease and iron deficiency anemia in human blood by Raman spectroscopy.Lasers in medical science · 2020Article
- Normal-subtracted preprocessing of Raman spectra aiming to discriminate skin actinic keratosis and neoplasias from benign lesions and normal skin tissues.Lasers in medical science · 2020Article
- Detecting creatine excreted in the urine of swimming athletes by means of Raman spectroscopy.Lasers in medical science · 2020Article
- Vibrational Spectroscopy Fingerprinting in Medicine: from Molecular to Clinical Practice.Materials (Basel, Switzerland) · 2019Review
- Biochemical changes in injured sciatic nerve of rats after low-level laser therapy (660 nm and 808 nm) evaluated by Raman spectroscopy.Lasers in medical science · 2019Article
- Discrimination model applied to urinalysis of patients with diabetes and hypertension aiming at diagnosis of chronic kidney disease by Raman spectroscopy.Lasers in medical science · 2017Article
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Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Raman spectroscopy has been employed in the quantitative analysis of biochemical components in human serum. This study aimed to develop a spectral model to estimate the concentration of glucose and lipid fractions in human serum, thus evaluating the feasibility of Raman spectroscopy technique for diagnostic purposes. A total of 44 samples of blood serum were collected from volunteers submitted to routine blood biochemical assay analysis. The biochemical concentrations of glucose, triglycerides, cholesterol, and high-density and low-density lipoproteins (HDL and LDL) were obtained by colorimetric method. Serum samples (200 μL) were submitted to Raman spectroscopy (830 nm, 250 mW, 50-s accumulation). The spectra of sera present peaks related to the main constituents, particularly proteins and lipids. A quantitative model based on partial least squares (PLS) regression has been developed to estimate the concentration of these compounds, taking the biochemical concentrations assayed by the colorimetric method as sample's actual concentrations. The PLS model based on leave-one-out cross-validation approach estimated the concentration of triglycerides and cholesterol with r = 0.98 and 0.96, and root mean square error of 35.4 and 15.9 mg/dL, respectively. For the other biochemicals, the r was ranging from 0.75 to 0.86. These results evidenced the possibility of performing biochemical assay in blood serum samples by Raman spectroscopy and PLS regression and may be employed as a means of diagnosis in routine clinical analysis.
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