ArticleBiomedical optics express2017
Time-stretch microscopy on a DVD for high-throughput imaging cell-based assay.
Article in Biomedical optics express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- High-speed laser-scanning biological microscopy using FACED.Nature protocols · 2021Article
- Ultrafast polarization bio-imaging based on coherent detection and time-stretch techniques.Biomedical optics express · 2018Article
- High-throughput imaging flow cytometry by optofluidic time-stretch microscopy.Nature protocols · 2018Article
- Label-free detection of cellular drug responses by high-throughput bright-field imaging and machine learning.Scientific reports · 2017Article
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Authors and funding
6 authors.
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Abstract
Cell-based assay based on time-stretch imaging is recognized to be well-suited for high-throughput phenotypic screening. However, this ultrafast imaging technique has primarily been limited to suspension-cell assay, leaving a wide range of solid-substrate assay formats uncharted. Moreover, time-stretch imaging is generally restricted to intrinsic biophysical phenotyping, but lacks the biomolecular signatures of the cells. To address these challenges, we develop a spinning time-stretch imaging assay platform based on the functionalized digital versatile disc (DVD). We demonstrate that adherent cell culture and biochemically-specific cell-capture can now be assayed with time-stretch microscopy, thanks to the high-speed DVD spinning motion that naturally enables on-the-fly cellular imaging at an ultrafast line-scan rate of >10MHz. As scanning the whole DVD at such a high speed enables ultra-large field-of-view imaging, it could be favorable for scaling both the assay throughput and content as demanded in many applications, e.g. drug discovery, and rare cancer cell screening.
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