Evidence map›Paper›PMID 28270968›Full record

ArticleBiomedical optics express2017

Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam.

Dongli Xu, Weibin Zhou, Leilei Peng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Fourier Multiplexed Fluorescence Lifetime Imaging.Methods in molecular biology (Clifton, N.J.) · 2021
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Dongli XuCollege of Optical Sciences, the University of Arizona, 1630 East University Blvd., Tucson, AZ 85721, USA.
Weibin ZhouDepartment of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan, MI 48109, USA.
Leilei PengCollege of Optical Sciences, the University of Arizona, 1630 East University Blvd., Tucson, AZ 85721, USA; Department of Molecular and Cell Biology, University of Arizona, 1007 E. Lowell Street, Tucson. AZ 85721, USA.

Funding

High-resolution multi-color FLIM-FRET tomography of whole organismR01EB015481 · NIBIB · UNIVERSITY OF ARIZONA · PI PENG, LEILEI · 2012 to 2015
$1.3M
NIBIB NIH HHS R01 EB015481
6 · The paper itself

Abstract

Fourier multiplexed FLIM (FmFLIM) tomography enables multiplexed 3D lifetime imaging of whole embryos. In our previous FmFLIM system, the spatial resolution was limited to 25 μm because of the trade-off between the spatial resolution and the imaging depth. In order to achieve cellular resolution imaging of thick specimens, we built a tomography system with dual-color Bessel beam. In combination with FmFLIM, the Bessel FmFLIM tomography system can perform parallel 3D lifetime imaging on multiple excitation-emission channels at a cellular resolution of 2.8 μm. The image capability of the Bessel FmFLIM tomography system was demonstrated by 3D lifetime imaging of dual-labeled transgenic zebrafish embryos.

Indexed as

(110.0110) Imaging systems(170.2520) Fluorescence microscopy(170.6280) Spectroscopy, fluorescence and luminescence(180.6900) Three-dimensional microscopy

Identifiers

PMID28270968
PMCPMC5330577

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.