ArticleFrontiers in plant science2017
MLPA-Based Analysis of Copy Number Variation in Plant Populations.
Article in Frontiers in plant science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Investigating Intraspecific Diversity of Metabolic Gene Clusters with MLPA and Synthetic Probes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Droplet Digital PCR Improves Detection ofInternational journal of molecular sciences · 2025Article
- The known structural variations in hearing loss and their diagnostic approaches: a comprehensive review.Molecular biology reports · 2025Review
- An Assay for Variable Fragment Length Allele-Specific Genotyping.Methods in molecular biology (Clifton, N.J.) · 2025Article
- CRISPR/Cas9- and Cas3-mediated modification of copy number variation in rice.Frontiers in genome editing · 2025Article
- Differences in the intraspecies copy number variation of Arabidopsis thaliana conserved and nonconserved miRNA genes.Functional & integrative genomics · 2023Article
- Analysis of Arabidopsis non-reference accessions reveals high diversity of metabolic gene clusters and discovers new candidate cluster members.Frontiers in plant science · 2023Article
- Effective identification of CRISPR/Cas9-induced and naturally occurred mutations in rice using a multiplex ligation-dependent probe amplification-based method.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- AthCNV: A Map of DNA Copy Number Variations in the Arabidopsis Genome.The Plant cell · 2020Article
- Molecular diagnosis methods in familial hypercholesterolemia.Anatolian journal of cardiology · 2020Review
- Multiplex Ligation-Dependent Probe Amplification for Simultaneous Identification ofFrontiers in pharmacology · 2020Article
- Modeling copy number variation in the genomic prediction of maize hybrids.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Article
- Attention sports fans! The far-reaching contributions of bud sport mutants to horticulture and plant biology.Horticulture research · 2018Article
- Copy number variation and disease resistance in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Copy number variants (CNVs) are intraspecies duplications/deletions of large DNA segments (>1 kb). A growing number of reports highlight the functional and evolutionary impact of CNV in plants, increasing the need for appropriate tools that enable locus-specific CNV genotyping on a population scale. Multiplex ligation-dependent probe amplification (MLPA) is considered a gold standard in genotyping CNV in humans. Consequently, numerous commercial MLPA assays for CNV-related human diseases have been created. We routinely genotype complex multiallelic CNVs in human and plant genomes using the modified MLPA procedure based on fully synthesized oligonucleotide probes (90-200 nt), which greatly simplifies the design process and allows for the development of custom assays. Here, we present a step-by-step protocol for gene-specific MLPA probe design, multiplexed assay setup and data analysis in a copy number genotyping experiment in plants. As a case study, we present the results of a custom assay designed to genotype the copy number status of 12 protein coding genes in a population of 80
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