ArticleGenome biology2017
Post-transcriptional regulation of fruit ripening and disease resistance in tomato by the vacuolar protease SlVPE3.
Article in Genome biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 76 citations in OpenAlex.
- Methods for Inoculating Botrytis cinerea on Leaves and Fruit.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Regulation of the growth-to-ripening transition in tomato fruits by energy charge involving SlATP-PRT and SlAPRT1.Molecular horticulture · 2026Article
- The role of induced resistance in mitigating postharvest fungal diseases in fruits and vegetables.Journal of advanced research · 2026Review
- Protein post-translational modifications: key switches coordinating fruit ripening regulatory networks.Horticulture research · 2026Article
- Quorum quenching, lactic acid bacteria and microencapsulation as emerging and promising strategies to mitigate tomato soft rot.Frontiers in plant science · 2026Review
- MdMAPK6-mediated phosphorylation of MdWRKY9 regulates apple fruit ripening through interaction with MdERF5L.Horticulture research · 2025Article
- RD21 enhances resistance to the strawberry vein banding virus by promoting autophagy-mediated degradation of the viral silencing suppressor P6 protein.Plant biotechnology journal · 2025Article
- E3 ligase SlCOP1-1 stabilizes transcription factor SlOpaque2 and enhances fruit resistance to Botrytis cinerea in tomato.Plant physiology · 2024Article
- Beyond 'push-pull': unraveling the ecological pleiotropy of plant volatile organic compounds for sustainable crop pest management.Crop health · 2023Review
- The pivotal ripening gene SlDML2 participates in regulating disease resistance in tomato.Plant biotechnology journal · 2023Article
- Multiomics analysis reveals the molecular mechanisms underlying virulence in Rhizoctonia and jasmonic acid-mediated resistance in Tartary buckwheat (Fagopyrum tataricum).The Plant cell · 2023Article
- Involvement of Vacuolar Processing Enzyme CgVPE1 in Vacuole Rupture in the Programmed Cell Death during the Development of the Secretory Cavity inInternational journal of molecular sciences · 2023Article
- Current insights into posttranscriptional regulation of fleshy fruit ripening.Plant physiology · 2023Review
- Review
- SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato.Plant biotechnology journal · 2022Article
- Molecular mechanisms underlying multi-level defense responses of horticultural crops to fungal pathogens.Horticulture research · 2022Article
- Genome-wide binding analysis of the tomato transcription factor SlDof1 reveals its regulatory impacts on fruit ripening.Molecular horticulture · 2021Article
- Vacuolar Processing Enzymes Modulating Susceptibility Response toFrontiers in plant science · 2021Article
- Article
- Comparative Analysis ofFrontiers in nutrition · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundProteases represent one of the most abundant classes of enzymes in eukaryotes and are known to play key roles in many biological processes in plants. However, little is known about their functions in fruit ripening and disease resistance, which are unique to flowering plants and required for seed maturation and dispersal. Elucidating the genetic mechanisms of fruit ripening and disease resistance is an important goal given the biological and dietary significance of fruit.
resultsThrough expression profile analyses of genes encoding tomato (Solanum lycopersicum) cysteine proteases, we identify a number of genes whose expression increases during fruit ripening. RNA interference (RNAi)-mediated repression of SlVPE3, a vacuolar protease gene, results in alterations in fruit pigmentation, lycopene biosynthesis, and ethylene production, suggesting that SlVPE3 is necessary for normal fruit ripening. Surprisingly, the SlVPE3 RNAi fruit are more susceptible to the necrotrophic pathogen Botrytis cinerea. Quantitative proteomic analysis identified 314 proteins that differentially accumulate upon SlVPE3 silencing, including proteins associated with fruit ripening and disease resistance. To identify the direct SlVPE3 targets and mechanisms contributing to fungal pathogen resistance, we perform a screening of SlVPE3-interacting proteins using co-immunoprecipitation coupled with mass spectrometry. We show that SlVPE3 is required for the cleavage of the serine protease inhibitor KTI4, which contributes to resistance against the fungal pathogen B. cinerea.
conclusionsOur findings contribute to elucidating gene regulatory networks and mechanisms that control fruit ripening and disease resistance responses.
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