Evidence map›Paper›PMID 28270215›Full record

ArticleAIDS research and therapy2017

Characterization of occult hepatitis B virus infection among HIV positive patients in Cameroon.

George Gachara, Tshifhiwa Magoro, Lufuno Mavhandu, Emmaculate Lum, Helen K Kimbi, Roland N Ndip, Pascal O Bessong

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in AIDS research and therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 3 pooled it
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 3 syntheses or guidelines pooled it, 39 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Trial
  5. Occult hepatitis B infection among blood donors from Yaoundé, Cameroon.Blood transfusion = Trasfusione del sangue · 2019
    Trial
  6. Article
  7. Article
  8. Article
  9. Article
  10. Control of occult hepatitis B virus infection.Advances in laboratory medicine · 2022
    Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Occult hepatitis B virus infection in Sudan: A systematic review and meta-analysis.JGH open : an open access journal of gastroenterology and hepatology · 2020
    Review
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  18. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

George GacharaHIV/AIDS & Global Health Research Program, Department of Microbiology, University of Venda, Private Bag X5050, Thohoyandou, 0950, Limpopo, South Africa.
Tshifhiwa MagoroHIV/AIDS & Global Health Research Program, Department of Microbiology, University of Venda, Private Bag X5050, Thohoyandou, 0950, Limpopo, South Africa.
Lufuno MavhanduHIV/AIDS & Global Health Research Program, Department of Microbiology, University of Venda, Private Bag X5050, Thohoyandou, 0950, Limpopo, South Africa.
Emmaculate LumDepartment of Zoology and Animal Physiology, Faculty of Science, University of Buea, Buea, Cameroon.
Helen K KimbiDepartment of Zoology and Animal Physiology, Faculty of Science, University of Buea, Buea, Cameroon.
Roland N NdipDepartment of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, Alice, South Africa.
Pascal O BessongHIV/AIDS & Global Health Research Program, Department of Microbiology, University of Venda, Private Bag X5050, Thohoyandou, 0950, Limpopo, South Africa. bessong@univen.ac.za.
University of Venda · ZAUniversity of Buea · CMUniversity of Fort Hare · ZA

Funding

The Water and Health in Limpopo Innovations Fellowship ProgramD43TW009359 · FIC · UNIVERSITY OF VIRGINIA · PI GUERRANT, RICHARD L · 2012 to 2016
$1.7M
FIC NIH HHS D43 TW009359
6 · The paper itself

Abstract

purposeOccult hepatitis B infection (OBI) among HIV positive patients varies widely in different geographic regions. We undertook a study to determine the prevalence of occult hepatitis B infection among HIV infected individuals visiting a health facility in South West Cameroon and characterized occult HBV strains based on sequence analyses.

methodsPlasma samples (n = 337), which previously tested negative for hepatitis B surface antigen (HBsAg), were screened for antibodies against hepatitis B core (anti-HBc) and surface (anti-HBs) antigens followed by DNA extraction. A 366 bp region covering the overlapping surface/polymerase gene of HBV was then amplified in a nested PCR and the amplicons sequenced using Sanger sequencing. The resulting sequences were then analyzed for genotypes and for escape and drug resistance mutations.

resultsTwenty samples were HBV DNA positive and were classified as OBI giving a prevalence of 5.9%. Out of these, 9 (45%) were anti-HBs positive, while 10 (52.6%) were anti-HBc positive. Additionally, 2 had dual anti-HBs and anti-HBc reactivity, while 6 had no detectable HBV antibodies. Out of the ten samples that were successfully sequenced, nine were classified as genotype E and one as genotype A. Three sequences possessed mutations associated with lamivudine resistance. We detected a number of mutations within the major hydrophilic region of the surface gene where most immune escape mutations occur.

conclusionsFindings from this study show the presence of hepatitis B in patients without any of the HBV serological markers. Further prospective studies are required to determine the risk factors and markers of OBI.

Indexed as

AdolescentAdultBase SequenceCameroonChild, PreschoolCoinfectionCross-Sectional StudiesDNA, ViralDrug Resistance, ViralFemaleHepatitis BHepatitis B AntibodiesHepatitis B Core AntigensHepatitis B Surface AntigensHepatitis B virusHIVDNA, ViralHepatitis B AntibodiesHepatitis B Core AntigensHepatitis B Surface AntigensLamivudineCameroonHepatitis B virusHIVOccult hepatitis B infection

Identifiers

PMID28270215
PMCPMC5341455
OpenAlexW2592884309

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.