ArticleVirology journal2017
A rapid virus-induced gene silencing (VIGS) method for assessing resistance and susceptibility to cassava mosaic disease.
Article in Virology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 46 citations in OpenAlex.
- The Application of Virus-Induced Gene Silencing in the Study of Rose Petal Abscission.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Engineering VIGS Vectors by Modifying Movement Proteins of the 30K Family.Biotechnology journal · 2024Article
- Rapid and Visual Screening of Virus Infection in Sugar Beets Through Polerovirus-Induced Gene Silencing.Viruses · 2024Article
- Advancing virus-induced gene silencing in sunflower: key factors of VIGS spreading and a novel simple protocol.Plant methods · 2024Article
- Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava.Plant methods · 2023Article
- Mutations in DNA polymerase δ subunit 1 co-segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses.Nature communications · 2022Article
- Cassava shrunken-2 homolog MeAPL3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration.Plant molecular biology · 2022Article
- A cassava common mosaic virus vector for virus-induced gene silencing in cassava.Plant methods · 2021Article
- Stacking disease resistance and mineral biofortification in cassava varieties to enhance yields and consumer health.Plant biotechnology journal · 2021Article
- Article
- Biolistic Approach for Transient Gene Expression Studies in Plants.Methods in molecular biology (Clifton, N.J.) · 2020Review
- Article
- Gene silencing approaches through virus-based vectors: speeding up functional genomics in monocots.Plant molecular biology · 2019Review
- Multiple morphogenic culture systems cause loss of resistance to cassava mosaic disease.BMC plant biology · 2018Article
- Phloem-Triggered Virus-Induced Gene Silencing Using a Recombinant Polerovirus.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCassava mosaic disease (CMD) is a major constraint to cassava production in sub-Saharan Africa. Under field conditions, evaluation for resistance to CMD takes 12-18 months, often conducted across multiple years and locations under pressure from whitefly-mediated transmission. Under greenhouse or laboratory settings, evaluation for resistance or susceptibility to CMD involves transmission of the causal viruses from an infected source to healthy plants through grafting, or by using Agrobacterium-mediated or biolistic delivery of infectious clones. Following inoculation, visual assessment for CMD symptom development and recovery requires 12-22 weeks. Here we report a rapid screening system for determining resistance and susceptibility to CMD based on virus-induced gene silencing (VIGS) of an endogenous cassava gene.
resultsA VIGS vector was developed based on an infectious clone of the virulent strain of East African cassava mosaic virus (EACMV-K201). A sequence from the cassava (Manihot esculenta) ortholog of Arabidopsis SPINDLY (SPY) was cloned into the CP position of the DNA-A genomic component and used to inoculate cassava plants by Helios® Gene Gun microparticle bombardment. Silencing of Manihot esculenta SPY (MeSPY) using MeSPY1-VIGS resulted in shoot-tip necrosis followed by death of the whole plant in CMD susceptible cassava plants within 2-4 weeks. CMD resistant cultivars were not affected and remained healthy after challenge with MeSPY1-VIGS. Significantly higher virus titers were detected in CMD-susceptible cassava lines compared to resistant controls and were correlated with a concomitant reduction in MeSPY expression in susceptible plants.
conclusionsA rapid VIGS-based screening system was developed for assessing resistance and susceptibility to CMD. The method is space and resource efficient, reducing the time required to perform CMD screening to as little as 2-4 weeks. It can be employed as a high throughput rapid screening system to assess new cassava cultivars and for screening transgenic, gene edited and breeding lines under controlled growth conditions.
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