Evidence map›Paper›PMID 28268018›Full record

ArticleBiomaterials2017

Elastin-like protein-hyaluronic acid (ELP-HA) hydrogels with decoupled mechanical and biochemical cues for cartilage regeneration.

Danqing Zhu, Huiyuan Wang, Pavin Trinh, Sarah C Heilshorn, Fan Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.

0numbers the graph read from it
0cells of the map it votes in
71citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

71 citing papers in PubMed.

  1. Article
  2. On-Demand Chemically Degradable Hydrogels for Biological Applications.Chembiochem : a European journal of chemical biology · 2026
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11 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Danqing ZhuDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Huiyuan WangDepartment of Materials Science & Engineering, Stanford University, Stanford, CA 94305, USA.
Pavin TrinhDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Sarah C HeilshornDepartment of Materials Science & Engineering, Stanford University, Stanford, CA 94305, USA. Electronic address: heilshorn@stanford.edu.
Fan YangDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Orthopaedic Surgery, Stanford University, Stanford, CA 94305, USA. Electronic address: fanyang@stanford.edu.

Funding

Stanford/UNC Biomimetic U19 Research CenterU19AI116484 · NIAID · STANFORD UNIVERSITY · PI GREENBERG, HARRY BERNARD · 2015 to 2025
$13.5M
Microribbon scaffold-mediated Immunomodulation for Cranial Bone RepairR01DE024772 · NIDCR · STANFORD UNIVERSITY · PI YANG, FAN · 2015 to 2025
$4.6M
Engineered Intestinal Microenvironments as Preclinical Drug Screening PlatformsR21EB018407 · NIBIB · STANFORD UNIVERSITY · PI HEILSHORN, SARAH C · 2014 to 2015
$427k
NIAID NIH HHS U19 AI116484NIBIB NIH HHS R21 EB018407NIDCR NIH HHS R01 DE024772
6 · The paper itself

Abstract

Hyaluronic acid (HA) is a major component of cartilage extracellular matrix and is an attractive material for use as 3D injectable matrices for cartilage regeneration. While previous studies have shown the promise of HA-based hydrogels to support cell-based cartilage formation, varying HA concentration generally led to simultaneous changes in both biochemical cues and stiffness. How cells respond to the change of biochemical content of HA remains largely unknown. Here we report an adaptable elastin-like protein-hyaluronic acid (ELP-HA) hydrogel platform using dynamic covalent chemistry, which allows variation of HA concentration without affecting matrix stiffness. ELP-HA hydrogels were created through dynamic hydrazone bonds via the reaction between hydrazine-modified ELP (ELP-HYD) and aldehyde-modified HA (HA-ALD). By tuning the stoichiometric ratio of aldehyde groups to hydrazine groups while maintaining ELP-HYD concentration constant, hydrogels with variable HA concentration (1.5%, 3%, or 5%) (w/v) were fabricated with comparable stiffness. To evaluate the effects of HA concentration on cell-based cartilage regeneration, chondrocytes were encapsulated within ELP-HA hydrogels with varying HA concentration. Increasing HA concentration led to a dose-dependent increase in cartilage-marker gene expression and enhanced sGAG deposition while minimizing undesirable fibrocartilage phenotype. The use of adaptable protein hydrogels formed via dynamic covalent chemistry may be broadly applicable as 3D scaffolds with decoupled niche properties to guide other desirable cell fates and tissue repair.

Indexed as

AnimalsBiomarkersCartilageCattleCell ProliferationChondrocytesElastinExtracellular MatrixGene Expression RegulationGlycosaminoglycansHyaluronic AcidHydrogelsKineticsPhenotypeRegenerationBiomarkersElastinGlycosaminoglycansHyaluronic AcidHydrogelsAdaptable hydrogelsCartilage regenerationDynamic covalent chemistryElastin-like protein (ELP)Hyaluronic acid

Identifiers

PMID28268018
PMCPMC5772736

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.