ArticlePloS one2017
Decellularized scaffold of cryopreserved rat kidney retains its recellularization potential.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
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Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 50 citations in OpenAlex.
- Current strategies on kidney regeneration using tissue engineering approaches: a systematic review.BMC nephrology · 2025Pooled it
- Extracellular matrix of lung scaffolds submitted to different means of sterilization: a systematic review.F1000Research · 2024Pooled it
- Co-transplantation of pancreatic islets with adipose-derived mesenchymal stem cells in vascularized kidney ECM scaffolds to restore type 1 diabetes mellitus.Bioactive materials · 2026Article
- The Role of Biophysical Factors in Organ Development: Insights from Current Organoid Models.Bioengineering (Basel, Switzerland) · 2024Review
- Machine Perfusion and Bioengineering Strategies in Transplantation-Beyond the Emerging Concepts.Transplant international : official journal of the European Society for Organ Transplantation · 2024Review
- Extracellular matrix: the critical contributor to skeletal muscle regeneration-a comprehensive review.Inflammation and regeneration · 2023Review
- The application of composite scaffold materials based on decellularized vascular matrix in tissue engineering: a review.Biomedical engineering online · 2023Review
- Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different Protocols.Bioengineering (Basel, Switzerland) · 2023Article
- Capturing effects of blood flow on the transplanted decellularized nephron with intravital microscopy.Scientific reports · 2023Article
- Influence of Storage Conditions on Decellularized Porcine Conjunctiva.Bioengineering (Basel, Switzerland) · 2023Article
- Effect of Co-culture of mesenchymal stem cell and glomerulus endothelial cell to promote endothelialization under optimized perfusion flow rate in whole renal ECM scaffold.Materials today. Bio · 2022Article
- Three-Dimensional Bioprinting of Decellularized Extracellular Matrix-Based Bioinks for Tissue Engineering.Molecules (Basel, Switzerland) · 2022Review
- Role of extracellular matrix components and structure in new renal modelsFrontiers in physiology · 2022Review
- Mechanical characterization of native and sugar-modified decellularized kidneys.Journal of the mechanical behavior of biomedical materials · 2021Article
- Asthmatic Bronchial Matrices Determine the Gene Expression and Behavior of Smooth Muscle Cells in a 3D Culture Model.Frontiers in allergy · 2021Article
- Accounting for Material Changes in Decellularized Tissue with Underutilized Methodologies.BioMed research international · 2021Review
- Human ucMSCs seeded in a decellularized kidney scaffold attenuate renal fibrosis by reducing epithelial-mesenchymal transition via the TGF-β/Smad signaling pathway.Pediatric research · 2020Article
- Growing a new human kidney.Kidney international · 2019Review
- Induced Intermediate Mesoderm Combined with Decellularized Kidney Scaffolds for Functional Engineering Kidney.Tissue engineering and regenerative medicine · 2019Article
- [Preparation of rat uterine decellularized scaffold and extracellular matrix hydrogel].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The multi-cellular nature of renal tissue makes it the most challenging organ for regeneration. Therefore, till date whole organ transplantations remain the definitive treatment for the end stage renal disease (ESRD). The shortage of available organs for the transplantation has, thus, remained a major concern as well as an unsolved problem. In this regard generation of whole organ scaffold through decellularization followed by regeneration of the whole organ by recellularization is being viewed as a potential alternative for generating functional tissues. Despite its growing interest, the optimal processing to achieve functional organ still remains unsolved. The biggest challenge remains is the time line for obtaining kidney. Keeping these facts in mind, we have assessed the effects of cryostorage (3 months) on renal tissue architecture and its potential for decellularization and recellularization in comparison to the freshly isolated kidneys. The light microscopy exploiting different microscopic stains as well as immuno-histochemistry and Scanning electron microscopy (SEM) demonstrated that ECM framework is well retained following kidney cryopreservation. The strength of these structures was reinforced by calculating mechanical stress which confirmed the similarity between the freshly isolated and cryopreserved tissue. The recellularization of these bio-scaffolds, with mesenchymal stem cells quickly repopulated the decellularized structures irrespective of the kidneys status, i.e. freshly isolated or the cryopreserved. The growth pattern employing mesenchymal stem cells demonstrated their equivalent recellularization potential. Based on these observations, it may be concluded that cryopreserved kidneys can be exploited as scaffolds for future development of functional organ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.