ArticlePloS one2017
On the adhesion-cohesion balance and oxygen consumption characteristics of liver organoids.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- From gut to liver: organoids as platforms for next-generation toxicology assessment vehicles for xenobiotics.Stem cell research & therapy · 2025Review
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- Size-related variability of oxygen consumption rates in individual human hepatic cells.Lab on a chip · 2024Article
- A sense of proximity: Cell packing modulates oxygen consumption.APL bioengineering · 2023Article
- Advanced 3D Models of Human Brain Tissue Using Neural Cell Lines: State-of-the-Art and Future Prospects.Cells · 2023Review
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- Oxygen Biosensors and Control in 3D Physiomimetic Experimental Models.Antioxidants (Basel, Switzerland) · 2021Review
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- The Effect of Matrix Stiffness on Human Hepatocyte Migration and Function-An In Vitro Research.Polymers · 2020Article
- An Integrated In Vitro-In Silico Approach for Silver Nanoparticle Dosimetry in Cell Cultures.Annals of biomedical engineering · 2020Article
- Injectable Cardiac Cell Microdroplets for Tissue Regeneration.Small (Weinheim an der Bergstrasse, Germany) · 2020Article
- Functionalized Enzyme-Responsive Biomaterials to Model Tissue StiffeningFrontiers in bioengineering and biotechnology · 2020Article
- Allometric Scaling of physiologically-relevant organoids.Scientific reports · 2019Article
- Oxygen Consumption Characteristics in 3D Constructs Depend on Cell Density.Frontiers in bioengineering and biotechnology · 2019Article
- Mathematical Models of Organoid Cultures.Frontiers in genetics · 2019Review
- Experimental and Computational Methods for the Study of Cerebral Organoids: A Review.Frontiers in neuroscience · 2019Review
- Comparison of frequency and strain-rate domain mechanical characterization.Scientific reports · 2018Article
- Effect of substrate stiffness on hepatocyte migration and cellular Young's modulus.Journal of cellular physiology · 2018Article
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- Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Liver organoids (LOs) are of interest in tissue replacement, hepatotoxicity and pathophysiological studies. However, it is still unclear what triggers LO self-assembly and what the optimal environment is for their culture. Hypothesizing that LO formation occurs as a result of a fine balance between cell-substrate adhesion and cell-cell cohesion, we used 3 cell types (hepatocytes, liver sinusoidal endothelial cells and mesenchymal stem cells) to investigate LO self-assembly on different substrates keeping the culture parameters (e.g. culture media, cell types/number) and substrate stiffness constant. As cellular spheroids may suffer from oxygen depletion in the core, we also sought to identify the optimal culture conditions for LOs in order to guarantee an adequate supply of oxygen during proliferation and differentiation. The oxygen consumption characteristics of LOs were measured using an O2 sensor and used to model the O2 concentration gradient in the organoids. We show that no LO formation occurs on highly adhesive hepatic extra-cellular matrix-based substrates, suggesting that cellular aggregation requires an optimal trade-off between the adhesiveness of a substrate and the cohesive forces between cells and that this balance is modulated by substrate mechanics. Thus, in addition to substrate stiffness, physicochemical properties, which are also critical for cell adhesion, play a role in LO self-assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.