Evidence map›Paper›PMID 28266869›Full record

ArticleCellular reprogramming2017

Pig Induced Pluripotent Stem Cell-Derived Neural Rosettes Parallel Human Differentiation Into Sensory Neural Subtypes.

Robin L Webb, Amalia Gallegos-Cárdenas, Colette N Miller, Nicholas J Solomotis, Hong-Xiang Liu, Franklin D West, Steven L Stice

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular reprogramming, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Robin L Webb1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .
Amalia Gallegos-Cárdenas1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .
Colette N Miller2 Department of Animal and Dairy Science, Rhodes Center for Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia , Athens, Georgia .
Nicholas J Solomotis1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .
Hong-Xiang Liu1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .
Franklin D West1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .
Steven L Stice1 Regenerative Bioscience Center, University of Georgia , Rhodes Center for Animal and Dairy Science, Athens, Georgia .
University of Georgia · US

Funding

Combined Nanoparticle and Neural Stem Cell Therapies in a Pig Model of StrokeR01NS093314 · NINDS · UNIVERSITY OF GEORGIA · PI WEST, FRANKLIN D · 2015 to 2019
$2.4M
NINDS NIH HHS R01 NS093314
6 · The paper itself

Abstract

The pig is the large animal model of choice for study of nerve regeneration and wound repair. Availability of porcine sensory neural cells would conceptually allow for analogous cell-based peripheral nerve regeneration in porcine injuries of similar severity and size to those found in humans. After recently reporting that porcine (or pig) induced pluripotent stem cells (piPSCs) differentiate into neural rosette (NR) structures similar to human NRs, here we demonstrate that pig NR cells could differentiate into neural crest cells and other peripheral nervous system-relevant cell types. Treatment with either bone morphogenetic protein 4 or fetal bovine serum led to differentiation into BRN3A-positive sensory cells and increased expression of sensory neuron TRK receptor gene family: TRKA, TRKB, and TRKC. Porcine sensory neural cells would allow determination of parallels between human and porcine cells in response to noxious stimuli, analgesics, and reparative mechanisms. In vitro differentiation of pig sensory neurons provides a novel model system for neural cell subtype specification and would provide a novel platform for the study of regenerative therapeutics by elucidating the requirements for innervation following injury and axonal survival.

Indexed as

Cell DifferentiationRosette FormationAnimalsBiomarkersCells, CulturedHumansInduced Pluripotent Stem CellsNeural Stem CellsNeurogenesisSensory Receptor CellsSwineBiomarkersneural crest cellsneural developmentsensory neural cells

Identifiers

PMID28266869
PMCPMC6016089
OpenAlexW2592061373

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.