ArticleScientific reports2017
Actual fusion efficiency in the lipid mixing assay - Comparison between nanodiscs and liposomes.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Review
- ATG2A-mediated DAG transfer recruits DGAT2 for lipid droplet growth.Nature structural & molecular biology · 2025Article
- Synergistic Cancer Metabolic Therapy via Co-Delivery of 3-Bromopyruvate and Temozolomide with a Supramolecular Shuttle.ACS applied materials & interfaces · 2025Article
- Temporizin-1 Meets the Membranes: Probing Membrane Inser-Tion and Disruption Mechanisms.Antibiotics (Basel, Switzerland) · 2025Article
- The Lassa Virus Stable Signal Peptide Undergoes a Conformational Change to Aid Viral Fusion.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Host Immune Cell Membrane Deformability Governs the Uptake Route of Malaria-Derived Extracellular Vesicles.ACS nano · 2025Article
- Surface tension-driven sorting of human perilipins on lipid droplets.The Journal of cell biology · 2024Article
- Extracellular Vesicle and Lipoprotein Interactions.Nano letters · 2024Review
- The beginning and the end of SNARE-induced membrane fusion.FEBS open bio · 2022Review
- Modulating membrane fusion through the design of fusogenic DNA circuits and bilayer composition.Soft matter · 2022Article
- Cooperation of Conical and Polyunsaturated Lipids to Regulate Initiation and Processing of Membrane Fusion.Frontiers in molecular biosciences · 2021Article
- A lipid mixing assay to accurately quantify the fusion of outer membrane vesicles.Methods (San Diego, Calif.) · 2020Article
- Review
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2 authors.
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Abstract
Lipid exchange occurs between membranes during fusion or active lipid transfer. These processes are necessary in vivo for the homeostasis of the cell at the level of the membranes, the organelles and the cell itself. They are also used by the cell to interact with the surrounding medium. Several assays have been developed to characterize in vitro these processes on model systems. The most common one, relying on fluorescence dequenching, measures lipid mixing between small membranes such as liposomes or nanodiscs in bulk. Usually, relative comparisons of the rate of lipid exchange are made between measurements performed in parallel. Here, we establish a quantitative standardization of this assay to avoid any bias resulting from the temperatures, the chosen fluorescent lipid fractions and from the various detergents used to normalize the measurements. We used this standardization to quantitatively compare the efficiency of SNARE-induced fusion in liposome-liposome and liposome-nanodisc configurations having similar collision frequency. We found that the initial yield of fusion is comparable in both cases, 1 per 2-3 million collisions in spite of a much larger dequenching signal with nanodiscs. Also, the long-term actual fusion rate is slightly lower with nanodiscs than in the liposome-liposome assay.
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